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Fig. 4. Zebrafish Ae2 mediates Cl^–/Cl^– exchange. A: ³⁶Cl^– uptake into oocytes previously injected with water or with 3 or 10 ng cRNA encoding Ae2 or mAE2a. B: time course of ³⁶Cl^– efflux from representative oocytes previously injected with water () or with cRNA encoding Ae2 () or mAE2a (). Bath pH was 7.4 in the presence (ND-96) and absence of Cl^–. C: ³⁶Cl^– efflux rate constants of oocytes expressing Ae2 or mAE2a measured sequentially in ND-96, Cl^–-free medium, and finally in ND-96 containing 200 µM DIDS. D: DIDS concentration dependence of ³⁶Cl^– efflux rate constants in oocytes expressing Ae2 compared with mAE2a. Values in all bar graphs are means ± SE for (n) oocytes. Oocytes in B–D were injected with 2.7 ng ae2 cRNA or 3 ng of mAE2a cRNA. ³⁶Cl^– efflux rate constants in bar graphs reflect subtraction of values measured in water-injected oocytes.