Whether genetics may play a role in the pathophysiologic response of kidney tubules to oxalate exposure remains unexplored despite that as many as 15% of the U.S. population annually will experience a kidney stone composed of calcium oxalate. To explore this issue we utilized a panel of chromosome substitution strains (CSS) in which one chromosome at a time was transferred from the Brown Norway (BN) rat onto the Dahl salt-sensitive (SS) genetic background. Hyperoxaluria was induced by adding hydroxyproline (HP) to the drinking water. A dose response (0-2% HP) study found that both SS and BN exhibited the same level of oxalate excretion as HP concentration increased, but only the BN exhibited changes in urothelial pathology and demonstrated crystal deposition at sites of urothelial injury as a function of dose (at 1.5-2.0%). The consomic panel was treated with 2.0% HP and evaluated for hyperoxaluria, renal injury, and crystal deposition. Tubular injury (% Area) and crystal deposition (% Area) was similar between the resistant SS and SS-4, 6, 7, 8, 9, 11, 16 and 20^BN consomic rats. However, tubular injury was significantly increased in SS-2^BN compared to the SS parental (9.8%± 1.56 and 4.2%± 1.09, respectively). Crystal deposition was observed in SS-2^BN and SS-18^BN (4.7%± 0.70 and 3.5%± 1.3, respectively) to the same extent as seen in the susceptible BN (3.2%± 0.44). The fact that crystal deposition was observed in SS-18^BN without extensive overall tubule injury, compared to the more severe widespread tubular injury seen in SS-2^BN, suggests that the underlying mechanism of each locus is different. In conclusion, these studies establish that BN rats demonstrate oxalate associated pathology and they retain calcium oxalate crystals coincident with urothelial injury but SS rats do not. These observations establish that BN rat chromosome 2 and 18 harbor genes that contribute to these processes.