AJP - Renal Physiology, Vol 233, Issue 3 247-F252, Copyright © 1977 by American Physiological Society
Glycerol-induced acute renal failure in the two kidney Goldblatt rat
P. Churchill, A. Bidani, L. Fleischmann and B. Becker-McKenna
One to four weeks after the left renal artery was clipped and the
contralateral kidney was left untouched in Sprague-Dawley rats (two-kidney
Goldblatt preparation), the clips were removed under ether anesthesia and
10 ml/kg body wt of either 150 mM NaCl (control) or 50% glycerol in water
(experimental) were injected intramuscularly. The next day the rats were
anesthetized (sodium pentobarbital) and the renal function of both kidneys
was measured, after which the renal cortical renin content was measured by
incubation of tissue homogenate with angiotensinase-free rat renin
substrate. Radioimmunoassay was used to determine the rate of angiotensin I
production. Compared with controls, both kidneys of glycerol-injected rats
had reduced GFR (left 28, right 18% of controls), increased percentage of
fractional water excretion (left 5, right 6 times controls), and increased
percentage of fractional Na excretion (left 3, right 4 times controls).
Despite large differences in renal renin (left 28, 676, right 1,329 ng
angiotensin I/h per mg protein), the extent of renal failure produced by
glycerol was equal in the left and right kidneys. These results are
inconsistent with the hypothesis that renal renin content is directly
related to the severity of glycerol-induced renal failure in rats.
