AJP - Renal Physiology, Vol 238, Issue 2 99-106, Copyright © 1980 by American Physiological Society
Effect of DIDS on renal tubular transport
F. J. Koschier, M. F. Stokols, J. M. Goldinger, M. Acara and S. K. Hong
Two stilbene derivates that had been used to covalently label the Cl-
carrier in the erythrocyte were investigated for reactivity with the renal
organic anion system. These compounds,
4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) and
(4,4'-diisothiocyano)-dihydrostilbene-2,2'-disulfonate (H2DIDS), were found
to be potent inhibitors (Ki congruent to 35 microM) of p-aminohippurate
(PAH) transport in the renal cortical slice without affecting
tetraethylammonium (TEA) transport or tissue viability. During renal
clearance studies performed in the perfused kidney, DIDS decreased the
PAH/inulin clearance ratio to congruent to 1. When the possible renal
transport of [3H]H2DIDS was investigated, the renal slice transport or
binding of [3H]H2DIDS reached a slice-to-medium ratio of congruent to 6,
and this accumulation was decreased by probenecid. In perfused kidney
experiments, the [3H]H2DIDS/inulin clearance ratio was congruent to 0.8.
Since probenecid reduced this clearance ratio to congruent to 0.5, there
was the possibility that H2DIDS underwent tubular secretion. In conclusion,
DIDS and H2DIDS interacted with the renal organic anion transport system,
which indicated that these compounds were possible probes for this
transport system.
