AJP - Renal Physiology, Vol 245, Issue 2 254-F262, Copyright © 1983 by American Physiological Society
Effect of unilateral ureteral obstruction on metabolism of renal lipids in the rat
J. Tannenbaum, M. L. Purkerson and S. Klahr
The metabolism of lipids in the cortex and inner medulla of control and
experimental kidneys of rats with unilateral ureteral obstruction (UUO) was
studied in vitro. A marked increase in the tissue triglyceride content was
noted after 24 h of obstruction in the experimental kidney compared with
the contralateral kidney of rats with UUO and normal kidneys from
sham-operated rats. A decrease in total phospholipid content was seen in
the experimental kidney after 24 h of obstruction. The incorporation of
[1-14C]oleic acid or [14C]arachidonic acid into tissue triglycerides was
significantly increased in the cortex and medulla of the experimental
kidney of rats with UUO compared with either the contralateral kidney of
the same animals or kidneys from sham-operated rats. The oxidation of
[1-14C]oleic acid to 14CO2 and the incorporation of [14C]arachidonic acid
into tissue phospholipids or free fatty acids were significantly lower in
the experimental kidney of rats with UUO. The results strongly suggest an
increase in the net synthetic rate of triglycerides in the experimental
kidney of UUO rats. This increase may be related to both a decrease in
fatty acid oxidation and an increased release of fatty acids from
phospholipids presumably due to increased phospholipase activity.
