AJP - Renal Physiology, Vol 245, Issue 6 735-F742, Copyright © 1983 by American Physiological Society

ARTICLES

Effects of verapamil in models of ischemic acute renal failure in the rat

C. D. Malis, J. Y. Cheung, A. Leaf and J. V. Bonventre

This study was designed to determine whether verapamil protects renal function in experimental ischemia in the rat and, if so, whether the protection is mediated by verapamil's vasodilatory action or by an effect on renal cells independent of vascular perfusion. Inulin clearance (CIn) was examined for 3 h subsequent to 40 min of unilateral intrarenal infusion of norepinephrine (0.75 microgram X kg-1 X min-1) and 3 and 48 h subsequent to 40 min of unilateral renal pedicle clamp. In norepinephrine-induced ischemia CIn fell to 0.8 +/- 0.4% of preischemic values in saline-treated kidneys and 0.5 +/- 0.3% in verapamil post-treated kidneys. By contrast, CIn fell only to 52.3 +/- 6.5% of preischemic values in verapamil-pretreated kidneys. Verapamil pretreatment significantly counteracted the intrarenal vasoconstriction produced by norepinephrine, sustaining renal blood flow during the norepinephrine infusion. In pedicle clamp-induced ischemia verapamil pre- and posttreatment had no beneficial effect on preservation of glomerular filtration rate, whereas mannitol pretreatment was beneficial. Parallel studies in the isolated perfused rat kidney confirmed the in vivo observations. In conclusion, verapamil exerts no protective effect on renal function at 3 or 48 h when ischemia is induced by renal pedicle clamp. Likewise, verapamil administration subsequent to norepinephrine-induced ischemia is ineffective in preserving renal function. Verapamil pretreatment in norepinephrine-induced ischemia preserves renal function probably by attenuating the vasoconstrictive ischemic insult due to norepinephrine.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. Zuk and K. S. Matlin Induction of a laminin isoform and alpha 3beta 1-integrin in renal ischemic injury and repair in vivo Am J Physiol Renal Physiol, November 1, 2002; 283(5): F971 - F984. [Abstract] [Full Text] [PDF]

				A. Zuk, J. V. Bonventre, and K. S. Matlin Expression of fibronectin splice variants in the postischemic rat kidney Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1037 - F1053. [Abstract] [Full Text] [PDF]

				M. L. H. HONING, M. L. HIJMERING, D. E. BALLARD, Y. P. YANG, R. J. PADLEY, P. J. MORRISON, and T. J. RABELINK Selective ETA Receptor Antagonism with ABT-627 Attenuates All Renal Effects of Endothelin in Humans J. Am. Soc. Nephrol., August 1, 2000; 11(8): 1498 - 1504. [Abstract] [Full Text]

				A. Zuk, J. V. Bonventre, D. Brown, and K. S. Matlin Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney Am J Physiol Cell Physiol, September 1, 1998; 275(3): C711 - C731. [Abstract] [Full Text] [PDF]

				T. Ichimura, J. V. Bonventre, V. Bailly, H. Wei, C. A. Hession, R. L. Cate, and M. Sanicola Kidney Injury Molecule-1 (KIM-1), a Putative Epithelial Cell Adhesion Molecule Containing a Novel Immunoglobulin Domain, Is Up-regulated in Renal Cells after Injury J. Biol. Chem., February 13, 1998; 273(7): 4135 - 4142. [Abstract] [Full Text] [PDF]

				R. Thadhani, M. Pascual, and J. V. Bonventre Acute Renal Failure N. Engl. J. Med., May 30, 1996; 334(22): 1448 - 1460. [Full Text] [PDF]