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Am J Physiol Renal Physiol 248: F436-F443, 1985;
0363-6127/85 $5.00
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AJP - Renal Physiology, Vol 248, Issue 3 436-F443, Copyright © 1985 by American Physiological Society


ARTICLES

Defined human renal tubular epithelia in culture: growth, characterization, and hormonal response

P. D. Wilson, M. A. Dillingham, R. Breckon and R. J. Anderson

Individually microdissected nephron segments of defined epithelial origin from human kidneys were cultured in vitro in the present studies. Nephron segments of proximal convoluted tubule, proximal straight tubule, cortical thick ascending limb of Henle, and cortical collecting tubule were grown in defined media. Each cell type retained differentiated characteristics as assessed by ultrastructural morphology, marker enzyme profiles, and adenylate cyclase response to selected hormones. These studies demonstrate the feasibility of using primary cultures of well-defined segments of the human nephron to study human renal tubular epithelia in vitro.


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R. Rohatgi, L. Battini, P. Kim, S. Israeli, P. D. Wilson, G. L. Gusella, and L. M. Satlin
Mechanoregulation of intracellular Ca2+ in human autosomal recessive polycystic kidney disease cyst-lining renal epithelial cells
Am J Physiol Renal Physiol, April 1, 2008; 294(4): F890 - F899.
[Abstract] [Full Text] [PDF]




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