AJP - Renal Physiology, Vol 249, Issue 1 20-F27, Copyright © 1985 by American Physiological Society
Effect of barium on cell volume regulation in rabbit proximal straight tubules
P. A. Welling, M. A. Linshaw and L. P. Sullivan
Rabbit proximal straight tubules swell abruptly when exposed to hypotonic
medium but then shrink in a few minutes as they approach their base-line
volume following loss of solute and water. Potassium, the major
intracellular cation, as well as sodium, is lost during this process. In
the present experiments, we studied hypotonic cell volume regulation in the
presence of barium, an agent reported to decrease potassium permeability.
Exposure to BaCl2 significantly prolonged hypotonic volume recovery in a
dose-dependent manner. Tubules depleted of potassium and loaded with sodium
chloride by exposure to 10(-4) M ouabain for 1 h swelled osmometrically and
subsequently volume regulated in dilute medium. Volume regulation in such
tubules is a consequence of transbasement membrane hydrostatic forces. By
contrast, tubules similarly loaded with sodium, but also exposed to 10(-3)
M BaCl2, volume regulated only minimally in dilute medium, suggesting BaCl2
might also affect sodium movement. However, hypotonic volume regulation was
restored in sodium-loaded BaCl2-treated tubules when cells were more
effectively depleted of potassium by incubation in 0-mM potassium medium.
We conclude that barium retards hypotonic volume regulation primarily
because of its effect on potassium movement.
