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Am J Physiol Renal Physiol 249: F448-F455, 1985;
0363-6127/85 $5.00
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AJP - Renal Physiology, Vol 249, Issue 3 448-F455, Copyright © 1985 by American Physiological Society

ARTICLES

Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells

S. E. Guggino, B. A. Suarez-Isla, W. B. Guggino and B. Sacktor

Single channels in the apical cell membrane of primary cultured chick kidney cells were studied using the patch clamp technique. Cell-attached recordings revealed the presence of a 107 +/- 6 pS channel that increased fractional open time upon depolarization. Experiments with inside-out excised patches indicated that the channel is K+ selective, Ca2+ activated, and inhibited by Ba2+. The addition of forskolin or antidiuretic hormone (ADH) to the bath during cell-attached recordings caused an increase in the fractional open time of the channel. The activation of a K+ channel by increases in cAMP may be one way in which K+ secretion in the kidney is stimulated by ADH in vivo.




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