AJP - Renal Physiology, Vol 249, Issue 6 827-F831, Copyright © 1985 by American Physiological Society
Effect of urinary alkalinization on the intrarenal formation of kinins
J. Brukman, R. D. Murray, P. C. Churchill, A. G. Scicli and O. A. Carretero
Alterations in the urinary excretion rate of kallikrein (UKKV) have
frequently been assumed to reflect alterations in the intrarenal generation
of kinins. Since other factors such as urinary pH and the activity of renal
kininases may affect the intrarenal concentration of kinins, we
investigated the effect of urinary alkalinization on kinin excretion in the
presence and absence of kininase II inhibition. Urine was collected from
the ureters of rats during control (ctl) (0.15 M NaCl infusion) and
experimental (exp) periods. During exp periods, group I (time control) was
infused with 0.15 M NaCl, group II with 0.3 M NaCl, and groups III and IV
with 0.3 M NaHCO3, all at 0.12 ml/min. Prior to the ctl period, group IV
was pretreated with the kininase II inhibitor captopril (40 mg/kg). During
the exp period, urinary kinin excretion (UKiV) increased significantly in
all groups ([exp - ctl] UKiV = 21 +/- 7, 27 +/- 9, 52 +/- 14, and 70 +/- 9
pg X min-1 X kg-1 in groups I, II, III, and IV, respectively). Urinary pH
increased significantly in groups II, III, and IV. The increases in UKiV
and pH were significantly greater in the bicarbonate-infused rats than in
control. Partial correlation coefficients show that UKiV correlates with a
high degree of significance only with urinary pH (r = 0.6). During the ctl
period, UKiV in the captopril-pretreated rats was not different from that
in other groups.(ABSTRACT TRUNCATED AT 250 WORDS)
