AJP - Renal AJP: Renal Physiology
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Am J Physiol Renal Physiol 250: F379-F385, 1986;
0363-6127/86 $5.00
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AJP - Renal Physiology, Vol 250, Issue 3 379-F385, Copyright © 1986 by American Physiological Society

ARTICLES

Patch-clamp technique in renal physiology

L. G. Palmer

The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport. In this review, I briefly cover the basic concepts behind the patch-clamp technique and the kinds of information that can be obtained with it. I then summarize the applications of the technique to renal tissues and describe some of the channel types that have been observed to date in epithelia.




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