AJP - Renal Physiology, Vol 250, Issue 3 511-F515, Copyright © 1986 by American Physiological Society
Evidence that prostaglandin E2 stimulates chloride secretion in cultured A6 renal epithelial cells
R. Keeler and N. L. Wong
The effects of prostaglandin E2 (PGE2) on the transport of sodium and
chloride were studied in cultured A6 renal epithelial cells. PGE2 on the
basolateral but not the apical surface increased transmonolayer
short-circuit current (Isc) and conductance. These changes could not be
inhibited with amiloride or furosemide in the apical medium. Flux
measurements showed that although Isc and net flux of sodium were equal in
unstimulated cells, after addition of PGE2 the current increased with no
corresponding changes in bidirectional or net flux of sodium. Immersing the
cells in sodium-free or chloride-free media inhibited the effects of PGE2.
Measurements of the simultaneous fluxes of sodium and chloride showed that
after PGE2 was added there was a net flux of chloride from the basal to the
apical side (secretion) that was equal to the change in Isc. The effects of
PGE2 were inhibited by furosemide in the basal medium. We conclude that
PGE2 stimulates a process of chloride secretion in A6 cells.
