AJP - Renal Physiology, Vol 250, Issue 5 895-F900, Copyright © 1986 by American Physiological Society
Prostaglandin blockade impairs denervation diuresis and natriuresis in the rat
J. D. Barber, W. W. Harrington, N. G. Moss and C. W. Gottschalk
Acute unilateral renal denervation of control rats produced an ipsilateral
diuresis (5.5 +/- 0.8 to 10.0 +/- 1.0 microliter/min, P less than 0.01) and
natriuresis (579 +/- 202 to 2,668 +/- 225 neq/min, P less than 0.01)
without a significant change in glomerular filtration rate or effective
renal plasma flow. Inhibition of prostaglandin synthesis with indomethacin
or meclofenamate (4 mg/kg iv) after acute unilateral denervation eliminated
the diuresis (13.3 +/- 1.6 to 5.0 +/- 0.9 microliter/min, P less than 0.01)
and attenuated the natriuresis (3,098 +/- 462 to 1,097 +/- 163 neq/min, P
less than 0.01). Denervation diuresis and natriuresis were significantly
impaired to the same extent when denervation was performed after inhibition
of prostaglandin synthesis (3.2 +/- 0.3 to 4.9 +/- 0.4 microliter/min, NS;
and 490 +/- 154 to 1,036 +/- 274 neq/min, P less than 0.05 vs. control,
respectively). These results indicate that the natriuresis and diuresis
seen after acute unilateral denervation in anesthetized rats are highly
dependent upon prostaglandins and cannot be initiated or maintained when
prostaglandin synthesis is impaired by indomethacin or meclofenamate.
