AJP - Renal Physiology, Vol 250, Issue 6 1123-F1127, Copyright © 1986 by American Physiological Society
Production of platelet-activating factor in glomeruli and cultured glomerular mesangial cells
D. Schlondorff, P. Goldwasser, R. Neuwirth, J. A. Satriano and K. L. Clay
Platelet-activating factor (PAF;
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) results in contraction of
isolated glomeruli and cultured mesangial cells and concomitantly causes
release of arachidonic acid and prostaglandin E2 (PGE2) formation. The
kidney and isolated glomeruli can also generate material that has PAF
bioactivity. We therefore examined the capacity of isolated renal glomeruli
and cultured glomerular mesangial cells from rats to form PAF. Both
isolated glomeruli and cultured mesangial cells transformed
1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine ([3H]lyso-PAF) into a labeled
product comigrating both on thin-layer chromatography (TLC) and
high-pressure liquid chromatography (HPLC) with authentic PAF. Using rabbit
platelet aggregation as bioassay for PAF, we found that isolated glomeruli
produced 4 +/- 2 pmol/mg glomerular protein of PAF-like material, and
mesangial cells produced 30 +/- 8 pmol/mg cell protein when stimulated with
A23187 (10(-5) M) for 30 min. The major species of the PAF material
produced by mesangial cells was identified as
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine after HPLC separation,
followed by fast atom bombardment and gas chromatography-mass spectrometry.
These results show that glomerular mesangial cells can produce PAF, which
could contribute locally to the regulation of glomerular function.
