AJP - Renal Physiology, Vol 251, Issue 1 12-F16, Copyright © 1986 by American Physiological Society
Renin release from different parts of rat afferent arterioles in vitro
L. Baumbach and O. Skott
A technique was designed to study renin release from superfused rat
glomeruli with short attached arterioles (SAG), from single glomeruli with
long attached arterioles (LAG), and from single afferent arterioles (AA).
The preparations obtained by magnetic isolation and microdissection were
superfused individually, and the renin release was measured by an
ultramicroradioimmunoassay with a detection limit of 3 X 10(-9) Goldblatt
units. The renin content of one SAG was about one-fifth of that contained
in one AA. Isoprenaline (10(-5) M) did not change renin release from SAG,
whereas renin release from AA and LAG increased threefold (P less than
0.01). A 30-mosmol/kg reduction in medium sodium chloride concentration
increased renin release from SAG 50% (P less than 0.01). This challenge
caused no change in renin release from AA. It is concluded that the
isoprenaline-sensitive juxtaglomerular (JG) cells are located in the
afferent arteriole only at some distance from the glomerulus, whereas those
cells sensitive to sodium chloride are located within and/or close to the
glomerulus.
