AJP - Renal Physiology, Vol 251, Issue 4 627-F634, Copyright © 1986 by American Physiological Society
In vitro effects of angiotensin II on glomerular function
V. J. Savin
Effects of incubation with angiotensin II (ANG II) on isolated rat
glomeruli were studied to test the hypothesis that ANG II exerts a direct
effect on glomerular ultrafiltration coefficient (Kf) or hydraulic
conductivity (Lp). Glomeruli of adult Munich-Wistar rats were isolated in
isotonic medium and incubated at 37 degrees C in control medium or in
medium containing ANG II (5.3 X 10(-9) to 5.3 X 10(-6) M) for 20 min.
Glomerular volume decreased after incubation with ANG II. Relative volume
decrement of unselected cortical glomeruli averaged 6 +/- 3% in 5.3 X
10(-9) or 10(-8) M ANG II and 13 +/- 3% in 5.3 X 10(-6) M ANG II; volume of
superficial cortical glomeruli and deep cortical glomeruli diminished by 9
+/- 2 and 5 +/- 0.5%, respectively, after incubation with ANG II 5.3 X
10(-6) M. Glomerular oncometric response after equilibration with media of
differing protein concentrations was not altered by ANG II incubation. Kf
and Lp assessed in samples of glomeruli following ANG II incubation
averaged 4.5 +/- 0.5 nl X mm-1 X mmHg and 2.7 +/- 0.5 microliter X mm-1 X
mmHg X cm2, respectively, and were not significantly different from control
values (3.8 +/- 0.3 nl X mm-1 X mmHg and 2.4 +/- 0.3 microliter X mm-1 X
mmHg X cm2, respectively. In replicate studies of individual glomeruli
prior to and after ANG II incubation, Kf was also not significantly altered
(control Kf, 6.1 +/- 1.1; Kf after ANG II, 4.4 +/- 0.9 nl X min-1 X
mmHg).(ABSTRACT TRUNCATED AT 250 WORDS)
