AJP - Renal Physiology, Vol 252, Issue 1 109-F114, Copyright © 1987 by American Physiological Society
Glomerular angiotensin II receptor modulation in glycerol-induced acute renal failure
B. M. Wilkes and P. F. Mento
The objective of this study was to examine the relationship between plasma
angiotensin II (ANG II) and ANG II receptor binding to the glomerulus
following acute renal failure (ARF) induced by an injection of glycerol
(50%, 10 ml/kg im). At 2 and 24 h after glycerol injection, ARF was
documented by a rise in blood urea nitrogen (BUN) (normal, 18.6 +/- 0.7; 2
h after glycerol 29.3 +/- 2.3; 24 h after glycerol, 103.0 +/- 12.0 mg/dl; P
less than 0.002 each experimental vs. normal) and creatinine (normal, 0.41
+/- 0.02; 2 h after glycerol, 0.96 +/- 0.10; 24 h after glycerol, 2.40 +/-
0.30 mg/dl; P less than 0.001, experimental vs. normal groups). Plasma ANG
II rose from a control of 15.8 +/- 3.1 to 26.9 +/- 4.4 pg/ml at 2 h and
49.5 +/- 8.6 pg/ml at 24 h after glycerol (P less than 0.05, experimental
vs. normal). No changes in affinity or density of glomerular ANG II
receptors were detected either at 2 or 24 h after glycerol injection
despite the significant rise in plasma ANG II (glomerular ANG II receptors:
normal, 1,000 +/- 61; 2 h after glycerol, 896 +/- 120; 24 h after glycerol,
936 +/- 80 fmol/mg). The 24-h infusion of ANG II in normal rats to achieve
plasma levels identical to the 24 h ARF group caused ANG II receptor
density to fall to 58.1 +/- 8.6% of control values (P less than
0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
