AJP - Renal Physiology, Vol 252, Issue 6 977-F980, Copyright © 1987 by American Physiological Society
Effect of atrial natriuretic factor on renal function in rats with papillary necrosis
D. A. Hildebrandt and R. O. Banks
The current study was designed to evaluate whether the renal papilla
participates in the natriuretic and diuretic response to atrial natriuretic
factor (ANF). Papillary necrosis was induced in female rats by intravenous
infusion of 2-bromoethylamine hydrobromide (BEA) 48 h prior to clearance
experiments; untreated (UNT) animals served as controls. Rats were
anesthetized with pentobarbital sodium, tracheotomized, and catheters
placed in a femoral artery and vein and in the bladder. At the time of
surgery, the bladder urine was collected and its osmolality used as an
indication of papillary destruction. Mean urine osmolalities (+/- SE) of
BEA rats were significantly lower than those of UNT rats (443 +/- 10 vs.
1,229 +/- 57 mosmol/kg, respectively), indicating that BEA caused papillary
necrosis. Synthetic ANF (rat 8-33) was given as a bolus intravenously
(1.25, 2.5, 5.0 micrograms/kg). The ANF-induced increases in sodium
excretion were not significantly different between UNT and BEA rats at any
ANF dose. These results demonstrate that a functional papilla is not
required for the action of ANF in the rat.
