AJP - Renal Physiology, Vol 253, Issue 6 1164-F1170, Copyright © 1987 by American Physiological Society
Lack of neural control of atrial natriuretic peptide release in conscious dogs
Y. Nishida, A. Miyata, H. Morita, N. Uemura, K. Kangawa, H. Matsuo and H. Hosomi
Department of Physiology, Kagawa Medical School, Japan.
To determine whether or not cardiac nerves control the release of atrial
natriuretic polypeptide (ANP), we examined the effects of intrapericardial
cardiac denervation on circulating ANP in conscious dogs. Base-line level
of circulating ANP in six cardiac-denervated (CD) dogs (191 +/- 24 pg/ml)
was not significantly different from that in seven intact (INT) dogs (196
+/- 18 pg/ml). Three percent dextran-40 in saline (18 ml/kg, 20% of
estimated blood volume) was infused over 5 min, and the elevated left
atrial pressure (LAP; approximately 8 mmHg) was maintained by supplemental
infusion over 40 min. The increase in circulating ANP after infusion in the
CD dogs (462 +/- 61 pg/ml) was not significantly different from that in the
INT dogs (435 +/- 73 pg/ml). The regression line relating circulating ANP
(normalized with regard to the control value) with the change in LAP in the
INT dogs was not significantly different from that in the CD dogs. These
results suggest that the cardiac nerves do not control the tonic release of
ANP or the volume expansion-induced release of ANP.
