AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 254: F153-F158, 1988;
0363-6127/88 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Halevy, J.
Right arrow Articles by Hayslett, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Halevy, J.
Right arrow Articles by Hayslett, J. P.

AJP - Renal Physiology, Vol 254, Issue 1 153-F158, Copyright © 1988 by American Physiological Society

ARTICLES

Glucocorticoids have a different action than aldosterone on target tissue

J. Halevy, E. L. Boulpaep, M. E. Budinger, H. J. Binder and J. P. Hayslett
Department of Medicine, Yale School of Medicine, New Haven, Connecticut 06510.

Studies were performed, using a synthetic glucocorticoid, RU 28362, that has negligible binding to aldosterone receptors, to examine whether the effect of glucocorticoids on the electrical properties of rat distal colon involves a specific mechanism or crossover binding to aldosterone receptor sites. Compared with control, RU 28362, in a dose of 70 micrograms.100 g body wt-1.day-1, increased transepithelial potential differences (VT) twofold and the equivalent short-circuit current (ISC) threefold; epithelial resistance (RT) was reduced 25%. These changes were qualitatively similar to, but less than, the changes induced by the same dose of aldosterone. The effects of dexamethasone in the same dose were indistinguishable from the changes caused by aldosterone. The results of cell-impalement studies corresponded to whole epithelial changes; RU 28362 induced a small increase in basolateral potential difference, from -43 +/- 2 to -45 +/- 1 mV, and a fall in apical potential difference, from 40 +/- 2 to 36 +/- 1 mV, but these effects on cell membrane voltage were less than caused by either aldosterone or dexamethasone. In contrast to the marked inhibitory action of amiloride to reduce VT in aldosterone- and dexamethasone-treated animals, this sodium channel blocker had no effect on VT in RU 28362-treated animals. Similarly, while spironolactone caused marked inhibition of the changes in VT, RT, and ISC induced by aldosterone, the inhibitory action on tissue from RU 28362-treated animals was slight or absent. These results suggest that glucocorticoids have a specific aldosterone receptor-independent action on the electrical properties of the large intestine.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
I. Grotjohann, J.-D. Schulzke, and M. Fromm
Electrogenic Na+ transport in rat late distal colon by natural and synthetic glucocorticosteroids
Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G491 - G498.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online