AJP - Renal Physiology, Vol 255, Issue 6 1220-F1224, Copyright © 1988 by American Physiological Society

ARTICLES

Urinary cGMP as biological marker of the renal activity of atrial natriuretic factor

K. R. Wong, M. H. Xie, L. B. Shi, F. Y. Liu, C. L. Huang, D. G. Gardner and M. G. Cogan
Department of Medicine, University of California, San Francisco 94143.

Current evidence suggests guanosine 3',5'-cyclic monophosphate (cGMP) serves as the second messenger for atrial natriuretic factor (ANF) in the kidney in vivo. We examined whether extracellular cGMP accumulation quantitatively reflected the concentration of cGMP within renal cells and whether urinary excretion of cGMP correlated with the physiological action of ANF. cGMP egression was examined in renal epithelial LLC-PK1 cells. ANF augmented intracellular cGMP concentration and extracellular cGMP appearance. Extracellular cGMP was an excellent function of the time-integrated intracellular cGMP concentration. In clearance studies in awake rats, urinary cGMP was primarily of renal cellular origin and correlated with the natriuresis induced by ANF in a time-dependent and concentration-dependent fashion. Urinary cGMP excretion may be useful as a biological marker for the renal activity of ANF in vivo.

This article has been cited by other articles:

				F. Piquard, R. Richard, A. Charloux, S. Doutreleau, T. Hannedouche, G. Brandenberger, and B. Geny Hormonal, renal, hemodynamic responses to acute neutral endopeptidase inhibition in heart transplant patients J Appl Physiol, August 1, 2002; 93(2): 569 - 575. [Abstract] [Full Text] [PDF]

				T. E. Lohmeier, J. R. Lohmeier, J. F. Reckelhoff, and D. A. Hildebrandt Sustained influence of the renal nerves to attenuate sodium retention in angiotensin hypertension Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R434 - R443. [Abstract] [Full Text] [PDF]

				B. Geny, H. Hardy, J. Lonsdorfer, B. Eisenmann, P. Haberey, and F. Piquard Enhanced Natriuretic Response to Neutral Endopeptidase Inhibition in Heart-Transplant Recipients Hypertension, April 1, 1999; 33(4): 969 - 974. [Abstract] [Full Text] [PDF]

				S. R. Orth and E. Ritz The Nephrotic Syndrome N. Engl. J. Med., April 23, 1998; 338(17): 1202 - 1211. [Full Text] [PDF]

				X.-P. Ni, R. A. Kesterson, S. D. Sharma, V. J. Hruby, R. D. Cone, E. Wiedemann, and M. H. Humphreys Prevention of reflex natriuresis after acute unilateral nephrectomy by melanocortin receptor antagonists Am J Physiol Regulatory Integrative Comp Physiol, April 1, 1998; 274(4): R931 - R938. [Abstract] [Full Text] [PDF]

				J.-P. Valentin, W.-Z. Ying, W. G. Couser, and M. H. Humphreys Extrarenal resistance to atrial natriuretic peptide in rats with experimental nephrotic syndrome Am J Physiol Renal Physiol, March 1, 1998; 274(3): F556 - F563. [Abstract] [Full Text] [PDF]