AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 255: F1276-F1280, 1988;
0363-6127/88 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Engbretson, B. G.
Right arrow Articles by Stoner, L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Engbretson, B. G.
Right arrow Articles by Stoner, L. C.

AJP - Renal Physiology, Vol 255, Issue 6 1276-F1280, Copyright © 1988 by American Physiological Society

ARTICLES

The everted renal tubule: a methodology for direct assessment of apical membrane function

B. G. Engbretson, K. W. Beyenbach and L. C. Stoner
Department of Physiology, State University of New York Health Science Center, Syracuse 13210.

A technique is described whereby it is possible to evert a 0.5- to 1.0-mm segment of an amphibian renal tubule and perfuse it in vitro. Consequently, the apical membranes of an intact nephron fragment are directly accessible for electrophysiological study. Viability of the cells of everted diluting segments taken from Ambystoma kidney was indicated by 1) failure of the cells to take up trypan blue and 2) the existence of an apical membrane voltage (average 66 mV, cell negative), which decreased predictably in the presence of either 5 mM barium or elevated potassium in the luminal bathing solution. The utility of the everted tubule to patch clamp studies was tested. A large conductance channel that appeared to be selective for potassium could be demonstrated in a cell-attached patch of the apical membrane of an everted initial collecting tubule. The everted tubule preparation not only provides large quantities of apical membrane for patch clamp studies but, more importantly, allows the investigator to control the solutions bathing each membrane surface independently. The application of patch clamp techniques to perfused, everted tubules may then serve to more completely describe the role of the apical membrane in transcellular ion transport.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Y. Tallini and L. C. Stoner
Amiloride-sensitive sodium current in everted Ambystoma initial collecting tubule: short-term insulin effects
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1171 - C1181.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Rhinehart, Z. Zhang, and T. L. Pallone
Ca2+ signaling and membrane potential in descending vasa recta pericytes and endothelia
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F852 - F860.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. C. Stoner and S. C. Viggiano
Elevation of basolateral K+ induces K+ secretion by apical maxi K+ channels in Ambystoma collecting tubule
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1999; 276(2): R616 - R621.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online