| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 256, Issue 5 836-F842, Copyright © 1989 by American Physiological Society
ARTICLES |
D. A. Bushinsky
Nephrology Section, Pritzker School of Medicine, University of Chicago, Illinois 60637.
In vivo chronic metabolic acidosis induces bone mineral dissolution. Whether the dissolution is due to alterations in physicochemical factors alone, as in acute metabolic acidosis, or requires participation of bone cells is not clear. The effect of chronic respiratory acidosis on bone has also not been established. To determine the effects of chronic metabolic and respiratory acidosis on net calcium flux from bone, we cultured live and dead neonatal mouse calvariae for 99 h in control medium or in medium acidified (pH approximately equal to 7.1) either by lowering the bicarbonate concentration (Met) or by increasing the PCO2 (Resp). We measured net calcium flux (JCa) over 0-48, 48-96, and 96-99 h. Over the first 48 h, there was greater net calcium efflux from live and dead Met than from both Resp groups. All four acidic groups had greater net calcium efflux than controls. Over the last 51 h of the chronic 99 h culture, there was net calcium efflux only from live Met (JCa = 285 +/- 129 nmol.bone-1.3 h-1) and not from any of the other groups (live control, JCa = -183 +/- 24; live Resp, JCa = -110 +/- 22; dead control, JCa = -256 +/- 12; dead Met, JCa = 11 +/- 78; dead Resp, JCa = -27 +/- 47; each P less than 0.02 vs. live Met). There is net calcium efflux from live cultured neonatal mouse calvariae during chronic metabolic, but not respiratory, acidosis. During chronic acidosis, decreased medium bicarbonate, and not just a fall in pH, is necessary to enhance net calcium efflux from live bone.
This article has been cited by other articles:
|
|
 |
|
  K. K. Frick, K. LaPlante, and D. A. Bushinsky RANK ligand and TNF-{alpha} mediate acid-induced bone calcium efflux in vitro Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1005 - F1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lemann Jr., D. A. Bushinsky, and L. L. Hamm Bone buffering of acid and base in humans Am J Physiol Renal Physiol, November 1, 2003; 285(5): F811 - F832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bushinsky, S. B. Smith, K. L. Gavrilov, L. F. Gavrilov, J. Li, and R. Levi-Setti Chronic acidosis-induced alteration in bone bicarbonate and phosphate Am J Physiol Renal Physiol, September 1, 2003; 285(3): F532 - F539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maurer, W. Riesen, J. Muser, H. N. Hulter, and R. Krapf Neutralization of Western diet inhibits bone resorption independently of K intake and reduces cortisol secretion in humans Am J Physiol Renal Physiol, January 1, 2003; 284(1): F32 - F40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Krieger, W. R. Parker, K. M. Alexander, and D. A. Bushinsky Prostaglandins regulate acid-induced cell-mediated bone resorption Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1077 - F1082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Frick and D. A. Bushinsky In vitro metabolic and respiratory acidosis selectively inhibit osteoblastic matrix gene expression Am J Physiol Renal Physiol, November 1, 1999; 277(5): F750 - F755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Bushinsky, J. M. Chabala, K. L. Gavrilov, and R. Levi-Setti Effects of in vivo metabolic acidosis on midcortical bone ion composition Am J Physiol Renal Physiol, November 1, 1999; 277(5): F813 - F819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Frick and D. A. Bushinsky Chronic metabolic acidosis reversibly inhibits extracellular matrix gene expression in mouse osteoblasts Am J Physiol Renal Physiol, November 1, 1998; 275(5): F840 - F847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ashizawa, R. Fujimura, K. Tokuyama, and M. Suzuki A bout of resistance exercise increases urinary calcium independently of osteoclastic activation in men J Appl Physiol, October 1, 1997; 83(4): 1159 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Kraut and J. W. Coburn Bone, Acid, and Osteoporosis N. Engl. J. Med., June 23, 1994; 330(25): 1821 - 1822. [Full Text]
|
|
|
|
|
|
D. A. Bushinsky, W. R. Parker, K. M. Alexander, and N. S. Krieger Metabolic, but not respiratory, acidosis increases bone PGE2 levels and calcium release Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1058 - F1066. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
