AJP - Renal Physiology, Vol 256, Issue 5 909-F915, Copyright © 1989 by American Physiological Society

ARTICLES

Bradykinin receptors localized by quantitative autoradiography in kidney, ureter, and bladder

D. C. Manning and S. H. Snyder
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

We have localized high affinity [3H]bradykinin receptor binding sites by in vitro autoradiography in kidney, ureter, and bladder of the guinea pig. The peptide pharmacology of the binding sites corresponds to that of high affinity physiological bradykinin receptors previously described (Manning, D. C., R. Vavrek, J. M. Stewart, and S. H. Snyder. J. Pharmacol. Exp. Ther. 237:504-512, 1986). In the kidney, receptors are concentrated in the medulla with negligible binding in the cortex. Medullary receptors are localized to the interstitium just beneath the basal membrane of collecting tubule cells and between tubules. In the ureter and bladder, receptors are confined to the lamina propria just beneath the epithelial layer. Localizations in the kidney may relate to the diuretic and natriuretic actions of bradykinin. Ureteral and bladder receptors may be associated with a role of bradykinin in pain and inflammation.

This article has been cited by other articles:

				S.-H. Sivritas, D. W. Ploth, and W. R. Fitzgibbon Blockade of renal medullary bradykinin B2 receptors increases tubular sodium reabsorption in rats fed a normal-salt diet Am J Physiol Renal Physiol, September 1, 2008; 295(3): F811 - F817. [Abstract] [Full Text] [PDF]

				U. C. Kopp, D. M. Farley, M. Z. Cicha, and L. A. Smith Activation of renal mechanosensitive neurons involves bradykinin, protein kinase C, PGE2, and substance P Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2000; 278(4): R937 - R946. [Abstract] [Full Text] [PDF]