AJP - Renal Physiology, Vol 257, Issue 3 336-F340, Copyright © 1989 by American Physiological Society
Effects of atrial natriuretic peptide on renal medullary prostaglandin E2 production
R. J. Bolterman, M. D. Bentley, S. M. Sandberg, M. J. Fiksen-Olsen and J. C. Romero
Department of Physiology and Biophysics, Mayo Medical School, Rochester, Minnesota 55905.
Like arachidonic acid (AA) and bradykinin (BK), the intrarenal
administration of atrial natriuretic peptide (ANP) has been shown to
increase the urinary excretion of prostaglandin E2 (PGE2). In the present
study, the direct in vitro effects of ANP on PGE2 production were compared
with those of AA and BK. Canine renal inner medullary slices were
preincubated for 30 min and washed in aerated Krebs-Ringer buffer (37
degrees C). During the final incubation period, with the use of varied
concentrations of AA, BK, or ANP in Krebs-Ringer buffer, samples were
obtained at 0 and 30 min to be used for radioimmunoassay of PGE2. Although
the rate of PGE2 production was significantly increased 11-fold with AA and
threefold with BK, it was unaffected by four different doses of ANP (10(-5)
to 10(-11) M). Furthermore, the production of PGE2 during basal and
stimulated (BK or AA) conditions was significantly blocked by indomethacin
but not by ANP. These results indicate that ANP had no direct stimulatory
or inhibitory effect on the medullary production of PGE2.
