AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 257: F947-F952, 1989;
0363-6127/89 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sasaki, S.
Right arrow Articles by Marumo, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sasaki, S.
Right arrow Articles by Marumo, F.

AJP - Renal Physiology, Vol 257, Issue 6 947-F952, Copyright © 1989 by American Physiological Society

ARTICLES

Effects of carbonic anhydrase inhibitors on basolateral base transport of rabbit proximal straight tubule

S. Sasaki and F. Marumo
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

The effect of carbonic anhydrase inhibitors on basolateral base transport was examined in rabbit proximal straight tubules (S2 segment) perfused in vitro by double-barreled pH microelectrodes. Bath HCO3- reduction or Na+ removal induced an initial basolateral voltage (Vbl) depolarization followed by a late-phase depolarization. Administration of 1 mM acetazolamide (ACTZ) to the bath fluid caused a small inhibition of the initial depolarization, and a larger inhibition of the late phase depolarization. Bath HCO3- reduction decreased intracellular pH (pHi), and this pHi decrease was attenuated by ACTZ. Bath Na+ removal also decreased pHi, and this pHi decrease was completely blocked by ACTZ. However, a slow pHi decrease was observed in response to bath Na+ removal when the bath fluid contained ACTZ and the luminal fluid contained 4 mM amiloride. The addition of ACTZ or ethoxyzolamide to the bath caused a rapid Vbl hyperpolarization and pHi increase. These results demonstrate that carbonic anhydrase inhibitors inhibit basolateral Na(HCO3)n transport in intact rabbit proximal tubule cells (S2). The data suggest that at least one of the base species transported by the transporter is HCO3-, and cytosolic carbonic anhydrase is important in converting intracellular OH- to HCO3-.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Pushkin and I. Kurtz
SLC4 base (HCO3-, CO32-) transporters: classification, function, structure, genetic diseases, and knockout models
Am J Physiol Renal Physiol, March 1, 2006; 290(3): F580 - F599.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. Pickkers, R. S. Garcha, M. Schachter, P. Smits, and A. D. Hughes
Inhibition of Carbonic Anhydrase Accounts for the Direct Vascular Effects of Hydrochlorothiazide
Hypertension, April 1, 1999; 33(4): 1043 - 1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Breton, K. Hammar, P. J. S. Smith, and D. Brown
Proton secretion in the male reproductive tract: involvement of Cl--independent HCO-3 transport
Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1134 - C1142.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online