AJP - Renal Physiology, Vol 257, Issue 6 959-F966, Copyright © 1989 by American Physiological Society
Experimental Fanconi's syndrome resulting from 4-pentenoate infusion in the dog
A. Gougoux, N. Zan, D. Dansereau and P. Vinay
Renal Laboratory, Notre-Dame Hospital, Montreal, Quebec, Canada.
Studies were performed in anesthetized dogs to evaluate the effects of
4-pentenoate on urinary electrolyte excretion and renal metabolism. The
intravenous administration of 4-pentenoate (1 mumol.kg-1.min-1 during 180
min) markedly increased the urinary excretion of bicarbonate, phosphate,
potassium, amino acids, glucose, and various organic anions, whereas that
of sodium and chloride also rose but less strikingly. These results suggest
that 4-pentenoate markedly inhibits the proximal reabsorption of various
solutes and therefore reproduces an experimental Fanconi's syndrome.
Despite the rise in renal cortical concentration of alpha-ketoglutarate,
glutamine utilization and total ammonia production expressed per 100 ml
glomerular filtration rate increased following 4-pentenoate infusion, the
ammonia being diverted into the renal vein. This increment in glutamine
utilization was equal to the combined rise in the renal production of
glutamate and alpha-ketoglutarate. By contrast, renal lactate utilization
was drastically reduced. A causal relationship between the decreased renal
cortical ATP concentration and the inhibited reabsorption of various
solutes is suggested but cannot be established unequivocally.
