AJP - Renal Physiology, Vol 258, Issue 2 273-F280, Copyright © 1990 by American Physiological Society

ARTICLES

Chloride channels in the apical membrane of cortical collecting duct cells

D. B. Light, E. M. Schwiebert, G. Fejes-Toth, A. Naray-Fejes-Toth, K. H. Karlson, F. V. McCann and B. A. Stanton
Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03756.

Ion channels in the apical membrane of cortical collecting duct (CCD) cells in culture were studied by the patch-clamp technique. CCD cells from rabbit kidney were isolated by solid-phase immunoadsorption with a monoclonal antibody. The majority of CCD cells (93%) had phenotypic characteristics similar to intercalated cells (ICC). Although Cl- channels were present in the apical membrane of the ICC cells, they were rarely active in cell-attached patches; however, channels were activated after patch excision. In inside-out patches, the channels exhibited rapid flickering, substrates, and large unitary currents. The single-channel conductance was 303 pS, the Cl(-)-to-Na+ permeability ratio was 10:1 and the Cl(-)-to-HCO3- permeability ratio was 1.5:1. The channel was inhibited by the Cl- channel blockers 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, diphenylamine carboxylic acid, and 5-nitro-2-(3-phenylpropylamino)-benzoic acid. Although a reduction in the cytoplasmic Ca2+ concentration inhibited channel activity in both inside-out patches and cell-attached patches, alterations of Ca2+ within the physiological range did not change the channel open probability. Finally, changing the cytoplasmic pH (6.5 to 8.0) did not alter the open probability. Thus a large conductance anion channel is present in the apical membrane of CCD cells in culture. This channel may be involved in cell volume regulation or in Cl- and HCO3- secretion.

This article has been cited by other articles:

				A. H. Toychiev, R. Z. Sabirov, N. Takahashi, Y. Ando-Akatsuka, H. Liu, T. Shintani, M. Noda, and Y. Okada Activation of maxi-anion channel by protein tyrosine dephosphorylation Am J Physiol Cell Physiol, October 1, 2009; 297(4): C990 - C1000. [Abstract] [Full Text] [PDF]

				E. C. Y. Wang, J.-M. Lee, J. P. Johnson, T. R. Kleyman, R. Bridges, and G. Apodaca Hydrostatic pressure-regulated ion transport in bladder uroepithelium Am J Physiol Renal Physiol, October 1, 2003; 285(4): F651 - F663. [Abstract] [Full Text] [PDF]

				D. P. Wallace, M. Christensen, G. Reif, F. Belibi, B. Thrasher, D. Herrell, and J. J. Grantham Electrolyte and fluid secretion by cultured human inner medullary collecting duct cells Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1337 - F1350. [Abstract] [Full Text] [PDF]

				S. Tsuruoka and G. J. Schwartz Mechanisms of HCO-3 secretion in the rabbit connecting segment Am J Physiol Renal Physiol, October 1, 1999; 277(4): F567 - F574. [Abstract] [Full Text] [PDF]

				I. Rubera, M. Tauc, M. Bidet, C. Poujeol, B. Cuiller, A. Watrin, N. Touret, and P. Poujeol Chloride currents in primary cultures of rabbit proximal and distal convoluted tubules Am J Physiol Renal Physiol, November 1, 1998; 275(5): F651 - F663. [Abstract] [Full Text] [PDF]

				S. Breton, K. Hammar, P. J. S. Smith, and D. Brown Proton secretion in the male reproductive tract: involvement of Cl--independent HCO-3 transport Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1134 - C1142. [Abstract] [Full Text] [PDF]

				L. C. Martin, M. E. Hickman, C. M. Curtis, L. J. MacVinish, and A. W. Cuthbert Electrogenic bicarbonate secretion in mouse gallbladder Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1045 - G1052. [Abstract] [Full Text] [PDF]

				C. M. Curtis, L. C. Martin, C. F. Higgins, W. H. Colledge, M. E. Hickman, M. J. Evans, L. J. MacVinish, and A. W. Cuthbert Restoration by intratracheal gene transfer of bicarbonate secretion in cystic fibrosis mouse gallbladder Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1053 - G1060. [Abstract] [Full Text] [PDF]