AJP - Renal Physiology, Vol 258, Issue 2 397-F402, Copyright © 1990 by American Physiological Society

ARTICLES

Direct effects of endothelin in the rat kidney

T. Katoh, H. Chang, S. Uchida, T. Okuda and K. Kurokawa
Fourth Department of Internal Medicine, University of Tokyo, School of Medicine, Japan.

In the present study, we tested the direct effects of endothelin (ET) on rat kidney in vivo. ET was infused into the left renal artery of anesthetized rats at a rate of 0.5, 5, 20, or 40 pmol/h. ET reduced ipsilateral urine volume (V), clearance of inulin (CIN), and clearance of p-aminohippuric acid (CPAH) in a dose-dependent manner. Thus ET at 20 pmol/h did not change V but decreased renal plasma flow (RPF) and glomerular filtration rate (GFR) by 27.6 +/- 14.3 and 30.8 +/- 10.4%, respectively, in the ipsilateral kidney. ET at 0.5 pmol/h was without effect and at 5 pmol/h had only minor effects on CIN and CPAH of ipsilateral kidney. At 40 pmol/h, ET reduced ipsilateral V, GFR, and RPF by 52.3 +/- 21.4, 58.4 +/- 14.5, and 72.5 +/- 10.6%, respectively. Filtration fraction and fractional excretion of Na remained unchanged during ET infusion. ET, 40 pmol/h, infused into the renal artery together with atrial natriuretic peptide (ANP) at a rate of 12 pmol/h reduced the ipsilateral V, GFR, and RPF by 33.2 +/- 6.3, 26.1 +/- 6.0, and 27.2 +/- 7.1%, respectively, decrements less than those with ET alone. When a calcium-channel blocker nicardipine was infused at a rate of 2.5 micrograms/h into the renal artery together with ET, 20 pmol/h, there was little change in the ipsilateral V, RPF, and GFR; ET, 40 pmol/h, with nicardipine did not change V and decreased GFR and RPF by 25.9 +/- 5.6 and 23.1 +/- 10.8%, respectively, decrements less than those without nicardipine.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				B. Ponnuchamy and R. A. Khalil Cellular mediators of renal vascular dysfunction in hypertension Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1001 - R1018. [Abstract] [Full Text] [PDF]

				Z. Abassi, B. Bishara, T. Karram, S. Khatib, J. Winaver, and A. Hoffman Adverse effects of pneumoperitoneum on renal function: involvement of the endothelin and nitric oxide systems Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R842 - R850. [Abstract] [Full Text] [PDF]

				A. Hoffman, Z. A. Abassi, S. Brodsky, R. Ramadan, and J. Winaver Mechanisms of Big Endothelin-1-Induced Diuresis and Natriuresis : Role of ETB Receptors Hypertension, March 1, 2000; 35(3): 732 - 739. [Abstract] [Full Text] [PDF]

				W. Hildebrandt, A. Ottenbacher, M. Schuster, E. R. Swenson, and P. Bartsch Diuretic effect of hypoxia, hypocapnia, and hyperpnea in humans: relation to hormones and O2 chemosensitivity J Appl Physiol, February 1, 2000; 88(2): 599 - 610. [Abstract] [Full Text] [PDF]

				P. A. Modesti, I. Cecioni, A. Migliorini, A. Naldoni, A. Costoli, S. Vanni, and G. G. N. Serneri Increased renal endothelin formation is associated with sodium retention and increased free water clearance Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H1070 - H1077. [Abstract] [Full Text] [PDF]

				A. O. Oyekan and J. C. McGiff Cytochrome P-450-derived eicosanoids participate in the renal functional effects of ET-1 in the anesthetized rat Am J Physiol Regulatory Integrative Comp Physiol, January 1, 1998; 274(1): R52 - R61. [Abstract] [Full Text] [PDF]