AJP - Renal Physiology, Vol 258, Issue 3 741-F750, Copyright © 1990 by American Physiological Society

ARTICLES

Effect of angiotensin II and norepinephrine on isolated rat afferent and efferent arterioles

B. H. Yuan, J. B. Robinette and J. D. Conger
Department of Medicine, University of Colorado School of Medicine, Denver.

Differential sensitivity of the pre- and postglomerular arterial vessels to vasoconstrictor activity of angiotensin II (ANG II) and norepinephrine (NE) is controversial. To avoid the complex extravascular neurohumoral variables that may have accounted for different results in the intact rat kidney, an isolated arteriole technique was used to examine the dose responses of ANG II and NE on afferent (AA) and efferent arterioles (EA) from Sprague-Dawley rats. EA were more sensitive than AA to ANG II (EC50 = 3.2 +/- 1.8 x 10(-11) and 1.0 +/- 1.6 x 10(-9) M, respectively, P less than 0.001), whereas EC50 of both AA and EA to NE were similar (3.4 +/- 2.3 x 10(-8) and 1.4 +/- 2.6 x 10(-8) M, respectively). The dose-response curves of AA to ANG II were not different when perfused at different luminal pressures (90 and 30 mmHg). In contrast, EA were more sensitive to ANG II at 30 than at 90 mmHg (3.0 +/- 1.2 x 10(-11) and 5.0 +/- 1.8 x 10(-10) M, respectively, P less than 0.005). The EC50 of EA to NE was unaffected by similar changes in luminal pressures. The mean dose-response curves of AA to ANG II were the same with and without the addition of 10(-5) M indomethacin; however, in arterioles displaying a focal constriction pattern to ANG II the response became uniform. It is concluded that, in the isolated rat glomerular arterioles, EA are more sensitive to ANG II than AA, but both vessels respond similarly to NE. The decreased ANG II sensitivity in AA is not related to the higher in vivo pressure, and the attenuated response in AA does not appear to be mediated primarily through ANG II-stimulated vasodilator prostanoid activity. EA sensitivity to ANG II appears to be inversely related to lumen pressure.

This article has been cited by other articles:

				A. Patzak, A. Steege, E. Y. Lai, J. O. Brinkmann, E. Kupsch, N. Spielmann, A. Gericke, A. Skalweit, J. Stegbauer, P. B. Persson, et al. Angiotensin II response in afferent arterioles of mice lacking either the endothelial or neuronal isoform of nitric oxide synthase Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R429 - R437. [Abstract] [Full Text] [PDF]

				M. Hultstrom, E. Y. Lai, Z. Ma, O. Kallskog, A. Patzak, and A. E. G. Persson Adenosine triphosphate increases the reactivity of the afferent arteriole to low concentrations of norepinephrine Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2007; 293(6): R2225 - R2231. [Abstract] [Full Text] [PDF]

				H. Ideura, K. Hiromura, N. Hiramatsu, T. Shigehara, S. Takeuchi, M. Tomioka, T. Sakairi, S. Yamashita, A. Maeshima, Y. Kaneko, et al. Angiotensin II provokes podocyte injury in murine model of HIV-associated nephropathy Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1214 - F1221. [Abstract] [Full Text] [PDF]

				S. Hashimoto, J. W. Adams, K. E. Bernstein, and J. Schnermann Micropuncture determination of nephron function in mice without tissue angiotensin-converting enzyme Am J Physiol Renal Physiol, March 1, 2005; 288(3): F445 - F452. [Abstract] [Full Text] [PDF]

				R. W. Fallet, H. Ikenaga, J. P. Bast, and P. K. Carmines Relative contributions of Ca2+ mobilization and influx in renal arteriolar contractile responses to arginine vasopressin Am J Physiol Renal Physiol, March 1, 2005; 288(3): F545 - F551. [Abstract] [Full Text] [PDF]

				P. B. Hansen, S. Hashimoto, J. Briggs, and J. Schnermann Attenuated renovascular constrictor responses to angiotensin II in adenosine 1 receptor knockout mice Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2003; 285(1): R44 - R49. [Abstract] [Full Text] [PDF]

				L. M. Harrison-Bernard, A. K. Cook, M. I. Oliverio, and T. M. Coffman Renal segmental microvascular responses to ANG II in AT1A receptor null mice Am J Physiol Renal Physiol, March 1, 2003; 284(3): F538 - F545. [Abstract] [Full Text] [PDF]

				G. J. Wehbi, J. Zimpelmann, R. M. Carey, D. Z. Levine, and K. D. Burns Early streptozotocin-diabetes mellitus downregulates rat kidney AT2 receptors Am J Physiol Renal Physiol, February 1, 2001; 280(2): F254 - F265. [Abstract] [Full Text] [PDF]

				M. Salomonsson, K. Brannstrom, and W. J. Arendshorst alpha 1-Adrenoceptor subtypes in rat renal resistance vessels: in vivo and in vitro studies Am J Physiol Renal Physiol, January 1, 2000; 278(1): F138 - F147. [Abstract] [Full Text] [PDF]

				M. Salomonsson and W. J. Arendshorst Calcium recruitment in renal vasculature: NE effects on blood flow and cytosolic calcium concentration Am J Physiol Renal Physiol, May 1, 1999; 276(5): F700 - F710. [Abstract] [Full Text] [PDF]