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Am J Physiol Renal Physiol 259: F9-F17, 1990;
0363-6127/90 $5.00
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AJP - Renal Physiology, Vol 259, Issue 1 9-17, Copyright © 1990 by American Physiological Society

ARTICLES

Endothelium-derived relaxing factor influences renal vascular resistance

J. Radermacher, U. Forstermann and J. C. Frolich
Department of Clinical Pharmacology, Hannover Medical School, Federal Republic of Germany.

The mechanism of action of different vasodilators was investigated in the isolated perfused kidney of the rat. Acetylcholine (ACh, 10 nM-1 microM) and ATP (10 nM-1 microM), compounds known to relax isolated arteries in an endothelium-dependent fashion, caused concentration-dependent decreases in renal vascular resistance (RVR). Also, the endothelium-independent vasodilators verapamil (100 nM-10 microM), glyceryl trinitrate (GTN, 1-100 microM), and sodium nitroprusside (SNP, 1-100 microM) reduced RVR concentration dependently. Gossypol (10 microM, 5 min), an inhibitor of endothelium-derived relaxing factor (EDRF) production and/or release, increased basal RVR by 5% and significantly inhibited the vasodilator effects of ACh and ATP but had no effect on verapamil- or GTN-induced decreases in RVR. Methylene blue (MB) increased RVR dose dependently by up to 50%. About 50% of this effect could be antagonized with phentolamine (1 microM). MB abolished the relaxant response to ATP and attenuated the response to ACh. The dose-response curve of SNP was shifted to the right, and the relaxation to verapamil was slightly reduced. L-NG-methylarginine (100 microM) increased RVR by approximately 20%, and this effect was completely reversed by L-arginine (1 mM). N omega-nitro-L-arginine (100 microM) increased RVR by approximately 40% and attenuated the response to ATP but had no effect on the SNP-induced decrease in RVR. These results suggest that EDRF plays an important role in the regulation of RVR.


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