AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 259: F222-F226, 1990;
0363-6127/90 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sonnenberg, H.
Right arrow Articles by Wilson, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sonnenberg, H.
Right arrow Articles by Wilson, D. R.

AJP - Renal Physiology, Vol 259, Issue 2 222-F226, Copyright © 1990 by American Physiological Society

ARTICLES

In vivo microperfusion of inner medullary collecting duct in rats: effect of amiloride and ANF

H. Sonnenberg, U. Honrath and D. R. Wilson
Department of Physiology, University of Toronto, Ontario, Canada.

A method is described that allows perfusion of the inner medullary collecting duct (IMCD) of the rat kidney in situ and in vivo. Fine polyethylene catheters connected to a microperfusion pump were inserted into collecting ducts via the openings at the exposed papilla tip. Perfusate contained 22Na as well as [3H]inulin. During perfusion at 30 nl/min, urine was simultaneously collected. A decrease in the Na-to-inulin concentration ratio in the urinary sample, compared with the perfusate, was taken as indicating unidirectional efflux of Na from the perfused duct system. The effects of luminal amiloride (2 X 10(-4) M) or atrial natriuretic factor (ANF, 10(-8) M) were studied. Compared with control perfusions, both agonists reduced Na efflux from the IMCD to approximately 50%, indicating luminal sites of action. Combination of amiloride and ANF at their respective concentrations had no further effect. The lack of statistically significant additivity suggests, but does not prove, that ANF, administered from the luminal side, is able to block amiloride-sensitive Na channels in the apical membrane of IMCD cells.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Clerico, F. A. Recchia, C. Passino, and M. Emdin
Cardiac endocrine function is an essential component of the homeostatic regulation network: physiological and clinical implications
Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H17 - H29.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. BAILLY
Effect of Luminal Atrial Natriuretic Peptide on Chloride Reabsorption in Mouse Cortical Thick Ascending Limb: Inhibition by Endothelin
J. Am. Soc. Nephrol., October 1, 2000; 11(10): 1791 - 1797.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
K. S. Misono
Atrial Natriuretic Factor Binding to Its Receptor Is Dependent on Chloride Concentration : A Possible Feedback-Control Mechanism in Renal Salt Regulation
Circ. Res., June 9, 2000; 86(11): 1135 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H. H. Chen, J. A. Schirger, W. L. Chau, M. Jougasaki, O. Lisy, M. M. Redfield, P. T. Barclay, and J. C. Burnett Jr
Renal Response to Acute Neutral Endopeptidase Inhibition in Mild and Severe Experimental Heart Failure
Circulation, December 14, 1999; 100(24): 2443 - 2448.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
T. Ogawa, B. G. Bruneau, N. Yokota, M. L. Kuroski de Bold, and A. J. de Bold
Tissue-Specific Regulation of Renal and Cardiac Atrial Natriuretic Factor Gene Expression in Deoxycorticosterone Acetate–Salt Rats
Hypertension, December 1, 1997; 30(6): 1342 - 1347.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online