AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 260: F39-F45, 1991;
0363-6127/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okada, K.
Right arrow Articles by Schrier, R. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okada, K.
Right arrow Articles by Schrier, R. W.

AJP - Renal Physiology, Vol 260, Issue 1 39-F45, Copyright © 1991 by American Physiological Society

ARTICLES

Effects of extra- and intracellular pH on vascular action of arginine vasopressin

K. Okada, P. Tsai, V. A. Briner, C. Caramelo and R. W. Schrier
Department of Medicine, University of Colorado School of Medicine, Denver 80262.

Compared with control studies performed at an extra-cellular pH (pHe) of 7.4, when vascular smooth muscle cells (VSMC) were preincubated in an acid extracellular medium (pHe 7.0) for 60 min the maximal arginine vasopressin (AVP)-induced sustained cellular contraction was reduced, whereas preincubation in an alkaline extracellular medium (pHe 7.8) enhanced the vascular action of AVP. These changes in VSMC contraction were accompanied by a parallel decrease in AVP receptor affinity and cytosolic free calcium ([Ca2+]i) mobilization in the acid medium, increased AVP receptor affinity, and [Ca2+]i mobilization in alkaline medium. Because the changes in pHe were parallelled by changes in pHi, studies were performed in which only pHi was altered by administering the proton ionophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP) (10(-6) M for 10 min). CCCP inhibited VSMC contraction in the absence of changes in AVP receptor binding and AVP-induced [Ca2+]i mobilization but abolished the AVP-induced cellular alkalinization. The results therefore indicate that in a nonbicarbonate buffer alterations in pHe may modulate the vascular responses to AVP by altering hormone receptor affinity, [Ca2+]i mobilization, and pHi.


This article has been cited by other articles:


Home page
 FASEB J.Home page
N. L'HEUREUX, J.-C. STOCLET, F. A. AUGER, G. J.-L. LAGAUD, L. GERMAIN, and R. ANDRIANTSITOHAINA
A human tissue-engineered vascular media: a new model for pharmacological studies of contractile responses
FASEB J, February 1, 2001; 15(2): 515 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Das, K. R. Stenmark, L. J. Ruff, and E. C. Dempsey
Selected isozymes of PKC contribute to augmented growth of fetal and neonatal bovine PA adventitial fibroblasts
Am J Physiol Lung Cell Mol Physiol, December 1, 1997; 273(6): L1276 - L1284.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online