AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 260: F163-F169, 1991;
0363-6127/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muto, S.
Right arrow Articles by Asano, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muto, S.
Right arrow Articles by Asano, Y.

AJP - Renal Physiology, Vol 260, Issue 2 163-F169, Copyright © 1991 by American Physiological Society

ARTICLES

Site and mechanism of action of epidermal growth factor in rabbit cortical collecting duct

S. Muto, H. Furuya, K. Tabei and Y. Asano
Department of Nephrology, Jichi Medical School, Tochigi, Japan.

To examine the exact target cell and mechanism of action of epidermal growth factor (EGF) in the isolated cortical collecting duct from rabbit kidney, we compared electrical properties of collecting duct (CD) cells (principal cells) and intercalated (IC) cells in absence and presence of EGF at 10(-8) M. Differentiation of CD and IC cells was based on values of basolateral membrane voltage (Vb) and fractional apical membrane resistance (fRa). In CD cells, upon addition of EGF to bath, lumen-negative transepithelial voltage (VT) was decreased from -8.0 +/- 1.9 to -2.4 +/- 1.3 mV (n = 22, P less than 0.001), but Vb was little changed (from -85.1 +/- 2.8 to -83.1 +/- 2.7 mV, n = 19), indicating that EGF in bath mainly caused changes in apical membrane voltage. In addition, peritubular EGF increased transepithelial resistance (RT) from 132.9 +/- 15.8 to 153.8 +/- 18.4 omega.cm2 (n = 16, P less than 0.001) as well as fRa from 0.31 +/- 0.06 to 0.39 +/- 0.07 (n = 12, P less than 0.01). These actions of EGF were prevented by pretreatment with 50 microM luminal amiloride. Luminal EGF had no effects on VT, Vb, RT, or fRa of CD cells. In IC cells, upon addition of EGF to bath, neither Vb nor fRa was affected. From these results, we conclude that EGF acts on the CD cell at the basolateral border and inhibits mainly the amiloride-sensitive Na+ conductance in the apical membrane.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
I. E. Veizis and C. U. Cotton
Abnormal EGF-dependent regulation of sodium absorption in ARPKD collecting duct cells
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F474 - F482.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J.-P. Shen and C. U. Cotton
Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F57 - F64.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Muto
Potassium Transport in the Mammalian Collecting Duct
Physiol Rev, January 1, 2001; 81(1): 85 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Muto, Y. Asano, D. Seldin, and G. Giebisch
Basolateral Na+ pump modulates apical Na+ and K+ conductances in rabbit cortical collecting ducts
Am J Physiol Renal Physiol, January 1, 1999; 276(1): F143 - F158.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online