AJP - Renal Physiology, Vol 260, Issue 6 913-F920, Copyright © 1991 by American Physiological Society

ARTICLES

Localization of endothelin-like immunoreactivity in rat kidneys

B. M. Wilkes, M. Susin, P. F. Mento, C. M. Macica, E. P. Girardi, E. Boss and E. P. Nord
Department of Medicine, North Shore University Hospital, Manhasset, New York 11030.

The distribution of endothelin-like immunoreactivity was examined in normal rat kidneys using the immunoperoxidase technique. A specific polyclonal antibody to endothelin 1, which recognized endothelin 1 and its precursor molecule, big endothelin, was raised in rabbits. Immunoperoxidase staining revealed specific endothelin-like immunoreactivity in the renal cortex, medulla, and papilla. Immunostaining density was greatest in the renal papilla where staining was predominantly localized to the vasa rectae of the distal nephron segments. Cytoplasmic immunostaining was noted focally in collecting duct cells in the renal papilla. In the renal medulla, intense immunostaining was identified in the vasa rectae. Cortical immunostaining was localized to the endothelial surfaces of arcuate arteries, veins, arterioles and peritubular capillaries. Glomerular immunostaining followed a capillary loop distribution and appeared to be predominantly localized to endothelial cells with smaller amounts of reaction product overlying the mesangium. The most proximal portion of the proximal tubule brush border and papillary collecting duct epithelium demonstrated focal endothelin-like immunostaining. We conclude that endothelin-like immunoreactivity is widely distributed in renal tissue compatible with important autacrine and paracrine actions in the kidneys.

This article has been cited by other articles:

				U. C. Kopp, M. Z. Cicha, and L. A. Smith Differential effects of endothelin on activation of renal mechanosensory nerves: stimulatory in high-sodium diet and inhibitory in low-sodium diet Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1545 - R1556. [Abstract] [Full Text] [PDF]

				J. P. Granger Endothelin Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R298 - R301. [Full Text] [PDF]

				R Anand, D Harry, S Holt, P Milner, M Dashwood, D Goodier, M Jarmulowicz, and K Moore Endothelin is an important determinant of renal function in a rat model of acute liver and renal failure Gut, January 1, 2002; 50(1): 111 - 117. [Abstract] [Full Text] [PDF]

				B. A. Yard, E. Chorianopoulos, D. Herr, and F. J. van der Woude Regulation of endothelin-1 and transforming growth factor-{beta}1 production in cultured proximal tubular cells by albumin and heparan sulphate glycosaminoglycans Nephrol. Dial. Transplant., September 1, 2001; 16(9): 1769 - 1775. [Abstract] [Full Text] [PDF]

				T.-J. Hsieh, S.-R. Lin, Y.-J. Lee, S.-J. Shin, Y.-H. Lai, C.-H. Hsu, and J.-H. Tsai Increased renal medullary endothelin-1 synthesis in prehypertensive DOCA- and salt-treated rats Am J Physiol Renal Physiol, July 1, 2000; 279(1): F112 - F121. [Abstract] [Full Text] [PDF]

				E. Brochu, S. Lacasse-M., C. Moreau, M. Lebel, I. Kingma, J. H. Grose, and R. Lariviere Endothelin ETA receptor blockade prevents the progression of renal failure and hypertension in uraemic rats Nephrol. Dial. Transplant., August 1, 1999; 14(8): 1881 - 1888. [Abstract] [Full Text] [PDF]

				C. Pupilli, P. Romagnani, L. Lasagni, F. Bellini, N. Misciglia, N. Emoto, M. Yanagisawa, M. Rizzo, M. Mannelli, and M. Serio Localization of endothelin-converting enzyme-1 in human kidney Am J Physiol Renal Physiol, November 1, 1997; 273(5): F749 - F756. [Abstract] [Full Text] [PDF]