AJP - Renal Physiology, Vol 260, Issue 6 921-F928, Copyright © 1991 by American Physiological Society

ARTICLES

Adenosine stimulates phosphate and glucose transport in opossum kidney epithelial cells

R. Coulson, R. A. Johnson, R. A. Olsson, D. R. Cooper and S. J. Scheinman
James A. Haley Veterans Hospital, Tampa, Florida 33612.

We have examined the effects of adenosine on sodium-coupled phosphate and glucose transport in cultured opossum kidney (OK) cells, a continuous cell line that resembles proximal tubule. Adenosine analogues R-(-)-N6-phenylisopropyladenosine (PIA) and 2',5'-dideoxy-adenosine (DDA) were employed as adenosine A1 receptor and P site-selective agonists, respectively. Sodium-dependent phosphate uptake activity (Na-Pi symport) increased by approximately 25% above both basal and parathyroid hormone (PTH)-inhibited levels in cells treated with PIA (0.1, 1 microM) but not in cells treated with DDA (100 microM). Adenosine (PIA) also stimulated sodium-coupled 3-O-methylglucose transport by approximately 40%. Intracellular adenosine 3',5'-cyclic monophosphate (cAMP) content was inversely related to Na-Pi symport activity in cells treated with PIA and PTH. However, changes in Na-Pi symport activity did not consistently relate to changes in intracellular cAMP. Protein kinase C was activated 15 s after treatment of OK cells with 1 microM PIA. Preincubation of cells with 3 microM staurosporine attenuated the effect of 1 microM PIA on phosphate uptake. These data suggest that Na-Pi and Na-glucose symport activities are stimulated by adenosine acting at a receptor coupled to more than one intracellular signal. It is likely that both protein kinases A and C are involved in these actions of adenosine.

This article has been cited by other articles:

				H. Castrop Modulation of adenosine receptor expression in the proximal tubule: a novel adaptive mechanism to regulate renal salt and water metabolism Am J Physiol Renal Physiol, July 1, 2008; 295(1): F35 - F36. [Full Text] [PDF]

				V. Vallon, B. Muhlbauer, and H. Osswald Adenosine and kidney function. Physiol Rev, July 1, 2006; 86(3): 901 - 940. [Abstract] [Full Text] [PDF]

				Y. J. Lee, S. H. Park, and H. J. Han ATP stimulates Na+-glucose cotransporter activity via cAMP and p38 MAPK in renal proximal tubule cells Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1268 - C1276. [Abstract] [Full Text] [PDF]

				E. K. Jackson, C. K. Kost Jr., W. A. Herzer, G. J. Smits, and S. P. Tofovic A1 Receptor Blockade Induces Natriuresis with a Favorable Renal Hemodynamic Profile in SHHF/Mcc-facp Rats Chronically Treated with Salt and Furosemide J. Pharmacol. Exp. Ther., December 1, 2001; 299(3): 978 - 987. [Abstract] [Full Text] [PDF]

				E. K. Jackson and R. K. Dubey Role of the extracellular cAMP-adenosine pathway in renal physiology Am J Physiol Renal Physiol, October 1, 2001; 281(4): F597 - F612. [Abstract] [Full Text] [PDF]

				C. S. WILCOX, W. J. WELCH, G. F. SCHREINER, and L. BELARDINELLI Natriuretic and Diuretic Actions of a Highly Selective Adenosine A1 Receptor Antagonist J. Am. Soc. Nephrol., April 1, 1999; 10(4): 714 - 720. [Abstract] [Full Text]

				A. C. Pflueger, T. J. Berndt, and F. G. Knox Effect of renal interstitial adenosine infusion on phosphate excretion in diabetes mellitus rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1228 - R1235. [Abstract] [Full Text] [PDF]