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AJP - Renal Physiology, Vol 261, Issue 1 76-F84, Copyright © 1991 by American Physiological Society
ARTICLES |
J. M. Chabala, R. Levi-Setti and D. A. Bushinsky
Enrico Fermi Institute, Department of Physics, University of Chicago, Illinois 60637.
Acidosis produced by a fall in [HCO3-] (metabolic acidosis, Met) produces greater Ca efflux from cultured bone than that produced by a rise in PCO2 (respiratory acidosis, Resp). To compare the effects of Met and Resp on bone surface ion composition we measured the surface abundance of 40Ca, 23Na, and 39K in cultured bone with a scanning ion microprobe utilizing secondary-ion mass spectrometry. Neonatal mouse calvariae were incubated for 24 h in medium simulating either Met (pH = 7.193 +/- 0.034, [HCO3-] = 15.1 +/- 1.4 meq/l), Resp (pH = 7.153 +/- 0.014, PCO2 = 85.4 +/- 1.2 mmHg) or normal physiological (Ctl; pH = 7.484 +/- 0.009, [HCO3-] = 29.7 +/- 0.7, PCO2 = 39.6 +/- 0.3) conditions. The surface of Ctl at 2-nm depth is rich in Na and K relative to Ca (Na/Ca = 25.6, K/Ca = 12.0, ratios of counts/s of secondary ions). Compared with Ctl, Met produced a sharp fall in both Na/Ca (6.5, P less than 0.01) and K/Ca (4.6, P less than 0.01), whereas Resp did not alter Na/Ca (23.8) or K/Ca (15.0). Ca efflux was greater in Met (873 +/- 54 nmol.bone-1.24 h-1) than in Resp (546 +/- 71 nmol.bone-1.24 h-1, P less than 0.01), which was greater than that in Ctl (315 +/- 49 nmol.bone-1.24 h-1, P less than 0.01 vs. Met and vs. Resp).(ABSTRACT TRUNCATED AT 250 WORDS)
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D. A. Bushinsky, S. B. Smith, K. L. Gavrilov, L. F. Gavrilov, J. Li, and R. Levi-Setti Acute acidosis-induced alteration in bone bicarbonate and phosphate Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1091 - F1097. [Abstract] [Full Text] [PDF]
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K. K. Frick and D. A. Bushinsky In vitro metabolic and respiratory acidosis selectively inhibit osteoblastic matrix gene expression Am J Physiol Renal Physiol, November 1, 1999; 277(5): F750 - F755. [Abstract] [Full Text] [PDF]
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D. A. Bushinsky, J. M. Chabala, K. L. Gavrilov, and R. Levi-Setti Effects of in vivo metabolic acidosis on midcortical bone ion composition Am J Physiol Renal Physiol, November 1, 1999; 277(5): F813 - F819. [Abstract] [Full Text] [PDF]
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D. A. Bushinsky, W. R. Parker, K. M. Alexander, and N. S. Krieger Metabolic, but not respiratory, acidosis increases bone PGE2 levels and calcium release Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1058 - F1066. [Abstract] [Full Text] [PDF]
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