AJP - Renal Physiology, Vol 261, Issue 5 741-F745, Copyright © 1991 by American Physiological Society
Absence of a regulatory role of angiotensin II in acute chloride-depletion alkalosis in rats
E. A. Walters, L. Rome, R. G. Luke and J. H. Galla
Division of Nephrology and Hypertension, University of Cincinnati College of Medicine, Ohio 45267.
Chloride-depletion alkalosis (CDA) has been characterized by hypereninemia.
To determine whether angiotensin II (ANG II) has an important role in its
maintenance or correction, anesthetized alkalotic rats, chloride depleted
by peritoneal dialysis, were infused with 5% dextrose and saralasin (1
microgram.kg-1.min-1) (SAR) or vehicle (SAR-C), 5% dextrose and
pretreatment with enalapril (1-1.5 mg/kg) (ENP) or vehicle (ENP-C), or 80
mM Cl solution with ANG II (20 micrograms/min) (ANG) or vehicle (ANG-C).
Rats infused with 5% dextrose showed no differences in the magnitude of the
alkalosis, inulin clearance, or urinary total CO2 excretion; both SAR and
ENP were associated with decreased blood pressure. In SAR, tCO2 delivery
out of late proximal convoluted tubule did not differ from that in SAR-C.
Rats infused with 80 mM Cl corrected CDA similarly (delta plasma [Cl] -
ANG-C + 6 +/- 1, ANG + 5 +/- 1 mM; P = not significant). These data suggest
that, although ANG II can importantly influence vascular tone and early
proximal tubule bicarbonate reabsorption, it does not have an important
role in the renal maintenance or correction of acute CDA.
