AJP - Renal Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 262: F1039-F1046, 1992;
0363-6127/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simpson, D. P.
Right arrow Articles by Zweifel, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simpson, D. P.
Right arrow Articles by Zweifel, K.

AJP - Renal Physiology, Vol 262, Issue 6 1039-F1046, Copyright © 1992 by American Physiological Society

ARTICLES

Substrate responses to acid-base alterations in dog renal proximal tubules

D. P. Simpson, N. McSherry, E. Scarbrough and K. Zweifel
Department of Medicine, University of Wisconsin-Madison 53792.

Proximal tubules from dog kidney were incubated for 2-6 min with low concentrations of pyruvate, glutamine, and malate. When initial medium citrate was between 0 and 0.5 mM and alpha-ketoglutarate was between 0 and 0.1 mM, concentrations of these two substrates in tubules and media after incubation were lower with 10 than with 40 mM HCO3-. Malate levels in tubules and media changed in the opposite direction. CO2 formation from labeled citrate or alpha-ketoglutarate was greater at low than at high HCO3-. In tubules treated with digitonin to disrupt the cell membrane, differences in citrate and alpha-ketoglutarate concentrations in tubules at high and low HCO3- were eliminated. The effects of acid-base changes seen in intact tubules on malate levels and on the rate of oxidation of labeled citrate persisted after digitonin pretreatment. These results show that acute effects of acid-base changes on citrate, alpha-ketoglutarate, and malate levels observed in intact renal cortex can be reproduced in isolated tubules. They suggest that these changes are related to changes in levels of citrate and alpha-ketoglutarate in cytoplasm without corresponding changes within mitochondria.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
S. H. Wright and W. H. Dantzler
Molecular and Cellular Physiology of Renal Organic Cation and Anion Transport
Physiol Rev, July 1, 2004; 84(3): 987 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Shuprisha, R. M. Lynch, S. H. Wright, and W. H. Dantzler
Real-time assessment of alpha -ketoglutarate effect on organic anion secretion in perfused rabbit proximal tubules
Am J Physiol Renal Physiol, October 1, 1999; 277(4): F513 - F523.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. R. Welborn, S. Shpun, W. H. Dantzler, and S. H. Wright
Effect of alpha -ketoglutarate on organic anion transport in single rabbit renal proximal tubules
Am J Physiol Renal Physiol, January 1, 1998; 274(1): F165 - F174.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online