| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 263, Issue 3 411-F416, Copyright © 1992 by American Physiological Society
ARTICLES |
P. Ernsberger, J. Zhou, T. H. Damon and J. G. Douglas
Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio 44106.
The selective angiotensin (ANG II) antagonists losartan (DuP 753) and PD 123319 have been shown to bind selectively to AT1 and AT2 subtypes, respectively. To characterize ANG II receptor subtypes in mesangial cells, washed membranes were incubated with 0.1 to 0.5 nM 125I-ANG II and increasing concentrations of competitors. The inhibition of 125I-ANG II binding by losartan and PD 123319 was biphasic, and LIGAND curve-fitting analysis revealed two populations of specific binding sites. One subpopulation comprised 86% of the total and showed high affinity for ANG II and losartan, but low affinity for the AT2 antagonists PD 123319 and CGP42112A, and thus appear identical to the recently cloned AT1 subtype. The remaining 14% of the sites showed nearly 100-fold lower affinity for losartan and 10,000-fold higher affinity for PD 123319 relative to AT1 sites. However, another AT2-selective antagonist, CGP42112A, showed little affinity for these sites. Both classes of binding sites were inhibited by guanosine 5'-O-(3-thiophosphate) and pertussis toxin treatment. We propose that there are two distinct G protein-coupled ANG II receptor subtypes (AT1A and AT1B) present in renal mesangial cells.
This article has been cited by other articles:
|
|
 |
|
  Y. Tan, F. N. Hutchison, and A. A. Jaffa Mechanisms of angiotensin II-induced expression of B2 kinin receptors Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H926 - H932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Park, C. Y. Song, B. C. Kim, H. K. Hong, and H. S. Lee Angiotensin II mediates LDL-induced superoxide generation in mesangial cells Am J Physiol Renal Physiol, November 1, 2003; 285(5): F909 - F915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Monti, M. Schinke, M. Bohm, D. Ganten, M. Bader, and G. Bricca Glial angiotensinogen regulates brain angiotensin II receptors in transgenic rats TGR(ASrAOGEN) Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R233 - R240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schwobel, T. Fischer, B. Lanz, and M. Mohaupt Angiotensin II receptor subtypes determine induced NO production in rat glomerular mesangial cells Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1092 - F1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Allred, M. C. Chappell, C. M. Ferrario, and D. I. Diz Differential actions of renal ischemic injury on the intrarenal angiotensin system Am J Physiol Renal Physiol, October 1, 2000; 279(4): F636 - F645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Handa Angiotensin-(1-7) can interact with the rat proximal tubule AT4 receptor system Am J Physiol Renal Physiol, July 1, 1999; 277(1): F75 - F83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jiao, X.-L. Cui, M. Torti, C.-H. Chang, L. D. Alexander, E. G. Lapetina, and J. G. Douglas Arachidonic acid mediates angiotensin II effects on p21ras in renal proximal tubular cells via the tyrosine kinase-Shc-Grb2-Sos pathway PNAS, June 23, 1998; 95(13): 7417 - 7421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sharma, R. Sharma, Andrew. S. Greene, E. T. McCarthy, and V. J. Savin Documentation of angiotensin II receptors in glomerular epithelial cells Am J Physiol Renal Physiol, March 1, 1998; 274(3): F623 - F627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ozono, Z.-Q. Wang, A. F. Moore, T. Inagami, H. M. Siragy, and R. M. Carey Expression of the Subtype 2 Angiotensin (AT2) Receptor Protein in Rat Kidney Hypertension, November 1, 1997; 30(5): 1238 - 1246. [Abstract] [Full Text]
|
|
|
|
|
|
A. Abbas, G. Gorelik, L. A. Carbini, and A. G. Scicli Angiotensin-(1-7) Induces Bradykinin-Mediated Hypotensive Responses in Anesthetized Rats Hypertension, August 1, 1997; 30(2): 217 - 221. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Oliveira, R. A.S. Santos, G. F.P. Santos, M. C. Khosla, and M. J. Campagnole-Santos Changes in the Baroreflex Control of Heart Rate Produced by Central Infusion of Selective Angiotensin Antagonists in Hypertensive Rats Hypertension, June 1, 1996; 27(6): 1284 - 1290. [Abstract] [Full Text]
|
|
|
|
|
|
D. Chansel, C. Llorens-Cortes, S. Vandermeersch, P. Pham, and R. Ardaillou Regulation of Angiotensin II Receptor Subtypes by Dexamethasone in Rat Mesangial Cells Hypertension, April 1, 1996; 27(4): 867 - 874. [Abstract] [Full Text]
|
|
|
|
|
|
R. A.S. Santos, A. C. Simoes e Silva, A. J. Magaldi, M. C. Khosla, K. R. Cesar, K. T. Passaglio, and N. C.V. Baracho Evidence for a Physiological Role of Angiotensin-(1-7) in the Control of Hydroelectrolyte Balance Hypertension, April 1, 1996; 27(4): 875 - 884. [Abstract] [Full Text]
|
|
|
|
|
|
M. Troyanovskaya, L. Song, G. Jayaraman, and D. P. Healy Expression of Aminopeptidase A, an Angiotensinase, in Glomerular Mesangial Cells Hypertension, March 1, 1996; 27(3): 518 - 522. [Abstract] [Full Text]
|
|
|
|
|
|
S. D. Hilchey and C. P. Bell-Quilley Association Between the Natriuretic Action of Angiotensin-(1-7) and Selective Stimulation of Renal Prostaglandin I2 Release Hypertension, June 1, 1995; 25(6): 1238 - 1244. [Abstract] [Full Text]
|
|
|
|
|
|
M. de Gasparo, A. Husain, W. Alexander, K. J. Catt, A. T. Chiu, M. Drew, T. Goodfriend, J. W. Harding, T. Inagami, and P. B.M.W.M. Timmermans Proposed Update of Angiotensin Receptor Nomenclature Hypertension, May 1, 1995; 25(5): 924 - 927. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
