AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 268: F793-F801, 1995;
0363-6127/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Terzi, F.
Right arrow Articles by Kleinknecht, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Terzi, F.
Right arrow Articles by Kleinknecht, C.

AJP - Renal Physiology, Vol 268, Issue 5 793-F801, Copyright © 1995 by American Physiological Society

ARTICLES

Subtotal but not unilateral nephrectomy induces hyperplasia and protooncogene expression

F. Terzi, C. Ticozzi, M. Burtin, V. Motel, H. Beaufils, D. Laouari, B. M. Assael and C. Kleinknecht
Institut National de la Sante et de la Recherche Medicale Unite 192, Hopital Necker, Enfants-Malades, Paris, France.

It is generally accepted that renal compensatory growth after unilateral nephrectomy (Uni) is due to prominent hypertrophy with no involvement of protooncogenes. Neither the balance between hypertrophy and hyperplasia nor the expression of the early-growth-related genes has been studied after subtotal nephrectomy (Nx). The occurrence of cystic tubular dilatations after Nx may suggest an excessive cell proliferation in this model. We measured DNA, RNA, and protein content, number of nuclei per tubular section, as well as c-fos, c-jun, c-myc, c-H-ras, c-sis, and c-erb-B2 protooncogene expression in kidneys taken at time of surgery and 2, 7, and 14 days after sham operation (control rats), Uni, or Nx. After Uni, hyperplasia was greater than expected (+79% for DNA at day 14) and was associated with moderate hypertrophy (+11% for protein/DNA ratio). After Nx, compensatory growth was due only to hyperplasia (+117% for DNA at day 14), with unchanged protein/DNA ratio (vs. Uni, P < 0.02). The greater hyperplasia after Nx was confirmed by nuclei counting. The protooncogene mRNA expression was constantly absent in control and Uni rats, whereas that of c-fos and c-jun genes was detected in Nx rats at day 14 with a 2- to 12-fold increment. The c-fos and c-jun protein levels were also increased at that time in Nx rats. This suggests the following: 1) the cellular events following Uni and Nx are not the same, and 2) the late protooncogene expression in Nx exclusively could favor a particular type of cell proliferation possibly more related with cystic formation than with actual compensatory growth.


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
E. Pillebout, M. Burtin, H. T. Yuan, P. Briand, A. S. Woolf, G. Friedlander, and F. Terzi
Proliferation and Remodeling of the Peritubular Microcirculation after Nephron Reduction : Association with the Progression of Renal Lesions
Am. J. Pathol., August 1, 2001; 159(2): 547 - 560.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
F. TERZI, M. BURTIN, and G. FRIEDLANDER
Using Transgenic Mice to Analyze the Mechanisms of Progression of Chronic Renal Failure
J. Am. Soc. Nephrol., November 1, 2000; 11(90002): 144S - 148.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Megyesi, P. M. Price, E. Tamayo, and R. L. Safirstein
The lack of a functional p21WAF1/CIP1 gene ameliorates progression to chronic renal failure
PNAS, September 14, 1999; 96(19): 10830 - 10835.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online