| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 268, Issue 5 854-F861, Copyright © 1995 by American Physiological Society
ARTICLES |
N. L. Kizer, D. Vandorpe, B. Lewis, B. Bunting, J. Russell and B. A. Stanton
Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Previously, we demonstrated that the mIMCD-K2 cell line, derived from the inner medullary collecting duct (IMCD) of a transgenic mouse, secretes Cl- by an electrogenic mechanism [N. L. Kizer, B. Lewis, and B. A. Stanton, Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F347-F355, 1995]. The objective of the present study was to characterize the cellular mechanisms of electrogenic Cl- secretion (IscCl) and to determine whether arginine vasopressin (AVP) and adenosine 3',5'-cyclic monophosphate (cAMP) stimulate IscCl. To this end, we measured IscCl across monolayers of mIMCD-K2 cells mounted in Ussing-type chambers. AVP increased IscCl with a Michaelis constant (Km) of 2.1 +/- 0.7 x 10(-12) M. 1-Desamino-8-D-AVP, a specific V2 receptor agonist, increased IscCl from 3.3 +/- 0.4 to 17.4 +/- 1.3 microA/cm2, 8-(4-Chlorophenylthio)-cAMP, a cell-permanent analogue of cAMP, a second messenger of AVP, increased IscCl from 1.4 +/- 0.3 to 15.2 +/- 1.2 microA/cm2. Furosemide and bumetanide, inhibitors of Na(+)-2Cl(-)-K+ cotransport, and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), an inhibitor of Cl-/HCO3- exchange, reduced IscCl when added to the basolateral solution. Our data suggest that AVP, via V2 receptors, and the second messenger cAMP stimulate IscCl and that Cl- secretion by mIMCD-K2 cells involves uptake of Cl- across the basolateral membrane by Na(+)-2Cl(-)-K+ cotransport and Cl-/HCO3- exchange and diffusion out of the cells across the apical membrane by cystic fibrosis transmembrane conductance regulator Cl- channels.
This article has been cited by other articles:
|
|
 |
|
  S. Soodvilai, Z. Jia, and T. Yang Hydrogen peroxide stimulates chloride secretion in primary inner medullary collecting duct cells via mPGES-1-derived PGE2 Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1571 - F1576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Olteanu, M. B. Hovater, and E. M. Schwiebert Intraluminal autocrine purinergic signaling within cysts: implications for the progression of diseases that involve encapsulated cyst formation Am J Physiol Renal Physiol, January 1, 2007; 292(1): F11 - F14. [Full Text] [PDF]
|
|
|
|
|
|
D. P. Wallace, G. Reif, A.-M. Hedge, J. B. Thrasher, and P. Pietrow Adrenergic regulation of salt and fluid secretion in human medullary collecting duct cells Am J Physiol Renal Physiol, October 1, 2004; 287(4): F639 - F648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Boese, O. Aziz, N. L. Simmons, and M. A. Gray Kinetics and regulation of a Ca2+-activated Cl- conductance in mouse renal inner medullary collecting duct cells Am J Physiol Renal Physiol, April 1, 2004; 286(4): F682 - F692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Wallace, L. A. Rome, L. P. Sullivan, and J. J. Grantham cAMP-dependent fluid secretion in rat inner medullary collecting ducts Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1019 - F1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Loffing, B. D. Moyer, D. Reynolds, and B. A. Stanton PBA increases CFTR expression but at high doses inhibits Cl- secretion in Calu-3 airway epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L700 - L708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. McCoy, A. L. Taylor, B. A. Kudlow, K. Karlson, M. J. Slattery, L. M. Schwiebert, E. M. Schwiebert, and B. A. Stanton Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors Am J Physiol Renal Physiol, October 1, 1999; 277(4): F552 - F559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takacs-Jarrett, W. E. Sweeney, E. D. Avner, and C. U. Cotton Morphological and functional characterization of a conditionally immortalized collecting tubule cell line Am J Physiol Renal Physiol, November 1, 1998; 275(5): F802 - F811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. SULLIVAN, D. P. WALLACE, and J. J. GRANTHAM Epithelial Transport in Polycystic Kidney Disease Physiol Rev, October 1, 1998; 78(4): 1165 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Loffing, B. D. Moyer, D. McCoy, and B. A. Stanton Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial cells Am J Physiol Cell Physiol, October 1, 1998; 275(4): C913 - C920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Breen, P. J. Mannon, and B. A. Benjamin Peptide YY inhibits vasopressin-stimulated chloride secretion in inner medullary collecting duct cells Am J Physiol Renal Physiol, September 1, 1998; 275(3): F452 - F457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Lee, C. M. Penland, J. H. Widdicombe, and J. J. Wine Evidence that Calu-3 human airway cells secrete bicarbonate Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L450 - L453. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
