AJP - Renal AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 269: F594-F600, 1995;
0363-6127/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehrich, R. W.
Right arrow Articles by Forrest, J. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehrich, R. W.
Right arrow Articles by Forrest, J. N., Jr

AJP - Renal Physiology, Vol 269, Issue 4 594-F600, Copyright © 1995 by American Physiological Society

ARTICLES

Tyrosine phosphorylation is a novel pathway for regulation of chloride secretion in shark rectal gland

R. W. Lehrich and J. N. Forrest Jr
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA.

We used the specific tyrosine kinase inhibitor genistein to define the involvement of tyrosine phosphorylation in the regulation of chloride transport in the rectal gland of the dogfish shark, a model for chloride secretion via a cystic fibrosis transmembrane conductance regulator (CFTR)-like channel. In the perfused gland, genistein (100 microM) promptly increased chloride secretion from basal values of 159 +/- 36 to 966 +/- 49 mueq.h-1.g-1 (P < 0.0001). Bumentanide fully reversed genistein-induced secretion. In primary culture monolayers of rectal gland tubular cells, genistein, but not the inactive 7-glucoside form, genistin, increased short-circuit current in a dose-dependent manner, from basal values of 2.7 +/- 4.3 to 104 +/- 10 microA/cm2 (P < 0.0001). Apically applied genistein induced significantly greater chloride secretion than basolateral addition. Genistein did not increase the adenosine 3',5'-cyclic monophosphate (cAMP) content of either perfused glands or cultured monolayers. Using an anti-phosphotyrosine antibody, we observed phosphorylation of multiple proteins. Four peptides, with molecular masses of 250, 210, 55, and 53 kDa, responded to genistein treatment with a decrease in tyrosine phosphorylation. These data demonstrate the following: 1) genistein induces bumetanide-sensitive chloride secretion in both perfused rectal glands and cultured tubular cells; 2) these effects are not accompanied by an elevation of tissue cAMP, indicating that genistein-induced secretion is not mediated by the cAMP-protein kinase A pathway; and 3) genistein-sensitive peptides are present in the rectal gland cell and are candidates for involvement in the regulation of chloride secretion.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. J. Baker and K. L. Hamilton
Genistein stimulates electrogenic Cl- secretion in mouse jejunum
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1636 - C1645.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. A. Goddard, M. J. Evans, and W. H. Colledge
Genistein activates CFTR-mediated Cl- secretion in the murine trachea and colon
Am J Physiol Cell Physiol, August 1, 2000; 279(2): C383 - C392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Luo, T. Zhu, A. Evagelidis, M. D. Pato, and J. W. Hanrahan
Role of protein phosphatases in the activation of CFTR (ABCC7) by genistein and bromotetramisole
Am J Physiol Cell Physiol, July 1, 2000; 279(1): C108 - C119.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. T. Blikslager, M. C. Roberts, K. M. Young, J. M. Rhoads, and R. A. Argenzio
Genistein augments prostaglandin-induced recovery of barrier function in ischemia-injured porcine ileum
Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G207 - G216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. Silva, R. J. Solomon, and F. H. Epstein
Mode of activation of salt secretion by C-type natriuretic peptide in the shark rectal gland
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1999; 277(6): R1725 - R1732.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. D. SCHULTZ, A. K. SINGH, D. C. DEVOR, and R. J. BRIDGES
Pharmacology of CFTR Chloride Channel Activity
Physiol Rev, January 1, 1999; 79(1): 109 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. Illek and H. Fischer
Flavonoids stimulate Cl conductance of human airway epithelium in vitro and in vivo
Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L902 - L910.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Keely, J. M. Uribe, and K. E. Barrett
Carbachol Stimulates Transactivation of Epidermal Growth Factor Receptor and Mitogen-activated Protein Kinase in T84 Cells. IMPLICATIONS FOR CARBACHOL-STIMULATED CHLORIDE SECRETION
J. Biol. Chem., October 16, 1998; 273(42): 27111 - 27117.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. He, S. Raman, Y. Guo, and W. W. Reenstra
Cystic fibrosis transmembrane conductance regulator activation by cAMP-independent mechanisms
Am J Physiol Cell Physiol, October 1, 1998; 275(4): C958 - C966.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. C. Hool, L. M. Middleton, and R. D. Harvey
Genistein Increases the Sensitivity of Cardiac Ion Channels to ß-Adrenergic Receptor Stimulation
Circ. Res., July 13, 1998; 83(1): 33 - 42.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online