| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 270, Issue 1 53-F60, Copyright © 1996 by American Physiological Society
ARTICLES |
H. P. Koster, A. Hartog, C. H. van Os and R. J. Bindels
Department of Cell Physiology, University of Nijmegen, The Netherlands.
Rabbit connecting tubule and cortical collecting duct cells were isolated by immunodissection and cultured to confluence on permeable filters and on glass coverslips. Extracellular ATP dose-dependently reduced transcellular Na+ and Ca2+ transport (half-maximal inhibitory concentration, IC50, of 0.5 +/- 0.2 and 3.2 +/- 0.5 microM), with a maximal inhibition of 57 +/- 5 and 43 +/- 4%, respectively. Purinergic receptor agonists inhibited transport with the following rank order of potency: UTP = ATP > ADP; this suggests involvement of P2u purinoceptors. ATP also caused a dose-dependent (50% effective dose, EC50, of 1.5 +/- 0.2 microM) transient increase in intracellular Ca2+ concentration ([Ca2+]i), which decreased to a sustained elevated level. In the absence of extracellular Ca2+, a similar Ca2+ transient occurred, but the sustained response was abolished. Preloading the cells with the Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) completely prevented the ATP-induced Ca2+ transients, but not the ATP-induced inhibition of Na+ and Ca2+ absorption. Activation of protein kinase C (PKC) by the cell-permeable diacylglycerol analogue, 1,2-dioctanoyl-en-glycerol, mimicked ATP-induced inhibition of Na+ and Ca2+ absorption. The inhibitory effects of ATP were no longer observed after culturing cells in the presence of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) for 5 days, which resulted in downregulation of cellular PKC activity.
This article has been cited by other articles:
|
|
 |
|
  S. S.P. Wildman, J. Marks, C. M. Turner, L. Yew-Booth, C. M. Peppiatt-Wildman, B. F. King, D. G. Shirley, W. Wang, and R. J. Unwin Sodium-Dependent Regulation of Renal Amiloride-Sensitive Currents by Apical P2 Receptors J. Am. Soc. Nephrol., April 1, 2008; 19(4): 731 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Vallon P2 receptors in the regulation of renal transport mechanisms Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, C. S. Wingo, and S.-L. Xia Downregulation of SGK1 by nucleotides in renal tubular epithelial cells Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1751 - F1757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Lee, S. H. Park, and H. J. Han ATP stimulates Na+-glucose cotransporter activity via cAMP and p38 MAPK in renal proximal tubule cells Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1268 - C1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Falin, I. E. Veizis, and C. U. Cotton A role for ERK1/2 in EGF- and ATP-dependent regulation of amiloride-sensitive sodium absorption Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1003 - C1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-L. Xia, L. Wang, M. N. Cash, X. Teng, R. A. Schwalbe, and C. S. Wingo Extracellular ATP-induced calcium signaling in mIMCD-3 cells requires both P2X and P2Y purinoceptors Am J Physiol Renal Physiol, August 1, 2004; 287(2): F204 - F214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Leipziger Control of epithelial transport via luminal P2 receptors Am J Physiol Renal Physiol, March 1, 2003; 284(3): F419 - F432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Woda, M. Leite Jr., R. Rohatgi, and L. M. Satlin Effects of luminal flow and nucleotides on [Ca2+]i in rabbit cortical collecting duct Am J Physiol Renal Physiol, September 1, 2002; 283(3): F437 - F446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamamoto and Y. Suzuki Role of luminal ATP in regulating electrogenic Na+ absorption in guinea pig distal colon Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G300 - G308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lehrmann, J. Thomas, S. J. Kim, C. Jacobi, and J. Leipziger Luminal P2Y2 Receptor-Mediated Inhibition of Na+ Absorption in Isolated Perfused Mouse CCD J. Am. Soc. Nephrol., January 1, 2002; 13(1): 10 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kunzelmann and M. Mall Electrolyte Transport in the Mammalian Colon: Mechanisms and Implications for Disease Physiol Rev, January 1, 2002; 82(1): 245 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Dai, H. S. Kang, D. Kerstan, G. Ritchie, and G. A. Quamme ATP inhibits Mg2+ uptake in MDCT cells via P2X purinoceptors Am J Physiol Renal Physiol, November 1, 2001; 281(5): F833 - F840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Danahay, L. Withey, C. T. Poll, S. F. J. van de Graaf, and R. J. Bridges Protease-activated receptor-2-mediated inhibition of ion transport in human bronchial epithelial cells Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1455 - C1464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Schwiebert and B. K. Kishore Extracellular nucleotide signaling along the renal epithelium Am J Physiol Renal Physiol, June 1, 2001; 280(6): F945 - F963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Dai, G. Ritchie, D. Kerstan, H. S. Kang, D. E. C. Cole, and G. A. Quamme Magnesium Transport in the Renal Distal Convoluted Tubule Physiol Rev, January 1, 2001; 81(1): 51 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mall, A. Wissner, T. Gonska, D. Calenborn, J. Kuehr, M. Brandis, and K. Kunzelmann Inhibition of Amiloride-Sensitive Epithelial Na+ Absorption by Extracellular Nucleotides in Human Normal and Cystic Fibrosis Airways Am. J. Respir. Cell Mol. Biol., December 1, 2000; 23(6): 755 - 761. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Castro, L. Barlow-Walden, T. Woodson, J. D. Kerecman, G. H. Zhang, and J. R. Martinez Ion Transport in an Immortalized Rat Submandibular Cell Line SMG-C6 Experimental Biology and Medicine, October 1, 2000; 225(1): 39 - 48. [Abstract] [Full Text]
|
|
|
|
|
|
M. Bidet, G. De Renzis, S. Martial, I. Rubera, M. Tauc, and P. Poujeol Extracellular ATP increases [CA2+]i in distal tubule cells. I. Evidence for a P2Y2 purinoceptor Am J Physiol Renal Physiol, July 1, 2000; 279(1): F92 - F101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rubera, M. Tauc, M. Bidet, C. Verheecke-Mauze, G. De Renzis, C. Poujeol, B. Cuiller, and P. Poujeol Extracellular ATP increases [Ca2+]i in distal tubule cells. II. Activation of a Ca2+-dependent Cl- conductance Am J Physiol Renal Physiol, July 1, 2000; 279(1): F102 - F111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. J. Hoenderop, P. H. G. M. Willems, and R. J. M. Bindels Toward a comprehensive molecular model of active calcium reabsorption Am J Physiol Renal Physiol, March 1, 2000; 278(3): F352 - F360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. van Baal, J. G. J. Hoenderop, M. Groenendijk, C. H. van Os, R. J. M. Bindels, and P. H. G. M. Willems Hormone-stimulated Ca2+ transport in rabbit kidney: multiple sites of inhibition by exogenous ATP Am J Physiol Renal Physiol, December 1, 1999; 277(6): F899 - F906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. McCoy, A. L. Taylor, B. A. Kudlow, K. Karlson, M. J. Slattery, L. M. Schwiebert, E. M. Schwiebert, and B. A. Stanton Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors Am J Physiol Renal Physiol, October 1, 1999; 277(4): F552 - F559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Devor and J. M. Pilewski UTP inhibits Na+ absorption in wild-type and Delta F508 CFTR-expressing human bronchial epithelia Am J Physiol Cell Physiol, April 1, 1999; 276(4): C827 - C837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. J. Hoenderop, A. Hartog, P. H. G. M. Willems, and R. J. M. Bindels Adenosine-stimulated Ca2+ reabsorption is mediated by apical A1 receptors in rabbit cortical collecting system Am J Physiol Renal Physiol, April 1, 1998; 274(4): F736 - F743. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
