| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 270, Issue 4 657-F668, Copyright © 1996 by American Physiological Society
ARTICLES |
J. Frokiaer, D. Marples, M. A. Knepper and S. Nielsen
Department of Clinical Physiology, Aarhus University Hospital, Denmark.
Polyuria after release of bilateral ureteral obstruction (BUO) is frequently seen in patients with urological disorders. In this study, we examined the effect of BUO and release of BUO on the expression of the vasopressin-regulated water channel aquaporin-2 (AQP-2) in rat kidney. Ureters were obstructed for 24 h in all experiments, and BUO was either not released or released for 24 or 48 h or 7 days. Each group of experimental rats were matched with sham-operated controls. One kidney was used for membrane fractionation and immunoblotting, whereas the contralateral was fixed for immunocytochemistry. Immunoblotting demonstrated a significant reduction in AQP-2 expression in inner medullar during 24 h of BUO to 26 +/- 8% (P < 0.001). Release of BUO was associated with immediate onset of a predominant osmotic-dependent polyuria. Forty-eight hours after release of BUO, the reduction in AQP-2 expression persisted (19 +/- 8%, P < 0.001), concurrent with a marked nonosmotic postobstructive polyuria, as determined by a significant reduction in free-water clearance (-50 +/- 7 vs. -85 +/- 10 microliters.min-1.kg-1, P < 0.05). Immunofluorescence and immunoelectron microscopy confirmed the reduced levels of AQP-2 in collecting duct principal cells. Seven days after release, the renal excretion of water and electrolytes had almost normalized. However, the downregulation of AQP-2 was not partly reversed (49 +/- 14%, P < 0.001), and, consistent with this, the urinary concentrating capacity was significantly reduced 7 days after release to a 18-h period of thirst. This strongly suggests that the persistent downregulation of AQP-2 is the cause of the slow recovery in concentration capacity. In conclusion, BUO and release of BUO were associated with a marked reduction in expression of AQP-2, coincident with the development and maintenance of postobstructive polyuria. Thus reduced AQP-2 levels may represent an important factor in the slow recovery from postobstructive diuresis.
This article has been cited by other articles:
|
|
 |
|
  G. Wang, C. Li, S. W. Kim, T. Ring, J. Wen, J. C. Djurhuus, W. Wang, S. Nielsen, and J. Frokiaer Ureter obstruction alters expression of renal acid-base transport proteins in rat kidney Am J Physiol Renal Physiol, August 1, 2008; 295(2): F497 - F506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fenton, L. Brond, S. Nielsen, and J. Praetorius Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney Am J Physiol Renal Physiol, September 1, 2007; 293(3): F748 - F760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, R. Norregaard, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction Am J Physiol Renal Physiol, July 1, 2007; 293(1): F333 - F341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, S. O. Topcu, M. Diget, H. Schweer, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 activity transiently contributes to increased water and NaCl excretion in the polyuric phase after release of ureteral obstruction Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1322 - F1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Eskild-Jensen, K. Thomsen, C. Rungo, L. S. Ferreira, L. F. Paulsen, Y. F. Rawashdeh, J. R. Nyengaard, S. Nielsen, J. C. Djurhuus, and J. Frokiaer Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction Am J Physiol Renal Physiol, March 1, 2007; 292(3): F921 - F929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Jensen, C. Li, H. A. Praetorius, R. Norregaard, S. Frische, M. A. Knepper, S. Nielsen, and J. Frokiaer Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1021 - F1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Robben, N. V. A. M. Knoers, and P. M. T. Deen Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water channel in nephrogenic diabetes insipidus. Am J Physiol Renal Physiol, August 1, 2006; 291(2): F257 - F270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, Y. Shi, W. Wang, C. Sardeli, T.-H. Kwon, K. Thomsen, T. Jonassen, J. C. Djurhuus, M. A. Knepper, S. Nielsen, et al. {alpha}-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction Am J Physiol Renal Physiol, February 1, 2006; 290(2): F384 - F396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction Am J Physiol Renal Physiol, August 1, 2005; 289(2): F322 - F333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, J. D. Klein, W. Wang, M. A. Knepper, S. Nielsen, J. M. Sands, and J. Frokiaer Altered expression of urea transporters in response to ureteral obstruction Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1154 - F1162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of major renal Na transporters in rats with bilateral ureteral obstruction and release of obstruction Am J Physiol Renal Physiol, November 1, 2003; 285(5): F889 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiar Downregulation of renal aquaporins in response to unilateral ureteral obstruction Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1066 - F1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiar Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction Am J Physiol Renal Physiol, January 1, 2003; 284(1): F155 - F166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. KIM, S. H. CHO, B. S. OH, C. H. YEUM, K. C. CHOI, K. Y. AHN, and J. LEE Diminished Renal Expression of Aquaporin Water Channels in Rats with Experimental Bilateral Ureteral Obstruction J. Am. Soc. Nephrol., October 1, 2001; 12(10): 2019 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, L. Isikay, J. G. Wen, D. Marples, J. C. Djurhuus, A. Stockwell, M. A. Knepper, S. Nielsen, et al. Downregulation of AQP1, -2, and -3 after ureteral obstruction is associated with a long-term urine-concentrating defect Am J Physiol Renal Physiol, July 1, 2001; 281(1): F163 - F171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. KIM, J.-U. LEE, M.-Y. NAH, D.-G. KANG, K.-Y. AHN, H.-S. LEE, and K.-C. CHOI Cisplatin Decreases the Abundance of Aquaporin Water Channels in Rat Kidney J. Am. Soc. Nephrol., May 1, 2001; 12(5): 875 - 882. [Abstract] [Full Text]
|
|
|
|
|
|
T. E. N. Jonassen, S. Christensen, T.-H. Kwon, S. Langhoff, N. Salling, and S. Nielsen Renal water handling in rats with decompensated liver cirrhosis Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1101 - F1109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. VALENTI, A. LAERA, G. PACE, G. ACETO, M. L. LOSPALLUTI, R. PENZA, F. P. SELVAGGI, M. L. CHIOZZA, and M. SVELTO Urinary Aquaporin 2 and Calciuria Correlate with the Severity of Enuresis in Children J. Am. Soc. Nephrol., October 1, 2000; 11(10): 1873 - 1881. [Abstract] [Full Text]
|
|
|
|
|
|
P. AGRE Aquaporin Water Channels in Kidney J. Am. Soc. Nephrol., April 1, 2000; 11(4): 764 - 777. [Full Text] [PDF]
|
|
|
|
|
|
T. E. N. Jonassen, D. Promeneur, S. Christensen, J. S. Petersen, and S. Nielsen Decreased vasopressin-mediated renal water reabsorption in rats with chronic aldosterone-receptor blockade Am J Physiol Renal Physiol, February 1, 2000; 278(2): F246 - F256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Junaid, L. Cui, S. B. Penner, and D. D. Smyth Regulation of Aquaporin-2 Expression by the alpha 2-Adrenoceptor Agonist Clonidine in the Rat J. Pharmacol. Exp. Ther., November 1, 1999; 291(2): 920 - 923. [Abstract] [Full Text]
|
|
|
|
|
|
P. FERNÁNDEZ-LLAMA, R. TURNER, G. DIBONA, and M. A. KNEPPER Renal Expression of Aquaporins in Liver Cirrhosis Induced by Chronic Common Bile Duct Ligation in Rats J. Am. Soc. Nephrol., September 1, 1999; 10(9): 1950 - 1957. [Abstract] [Full Text]
|
|
|
|
|
|
T.-H. Kwon, J. Frokiaer, P. Fernandez-Llama, M. A. Knepper, and S. Nielsen Reduced abundance of aquaporins in rats with bilateral ischemia-induced acute renal failure: prevention by alpha -MSH Am J Physiol Renal Physiol, September 1, 1999; 277(3): F413 - F427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. FERNÁNDEZ-LLAMA, P. ANDREWS, R. TURNER, S. SAGGI, J. DIMARI, T.-H. KWON, S. NIELSEN, R. SAFIRSTEIN, and M. A. KNEPPER Decreased Abundance of Collecting Duct Aquaporins in Post-Ischemic Renal Failure in Rats J. Am. Soc. Nephrol., August 1, 1999; 10(8): 1658 - 1668. [Abstract] [Full Text]
|
|
|
|
|
|
H. WEN, J. FRØKI&Aelig;R, T.-H. KWON, and S. NIELSEN Urinary Excretion of Aquaporin-2 in Rat Is Mediated by a Vasopressin-Dependent Apical Pathway J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1416 - 1429. [Abstract] [Full Text]
|
|
|
|
|
|
S. NIELSEN, T.-H. KWON, B. M. CHRISTENSEN, D. PROMENEUR, J. FRØKI&Aelig;R, and D. MARPLES Physiology and Pathophysiology of Renal Aquaporins J. Am. Soc. Nephrol., March 1, 1999; 10(3): 647 - 663. [Abstract] [Full Text]
|
|
|
|
|
|
D. Marples, J. Frokiaer, and S. Nielsen Long-term regulation of aquaporins in the kidney Am J Physiol Renal Physiol, March 1, 1999; 276(3): F331 - F339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frokiaer, D. Marples, H. Valtin, J. F. Morris, M. A. Knepper, and S. Nielsen Low aquaporin-2 levels in polyuric DI +/+ severe mice with constitutively high cAMP-phosphodiesterase activity Am J Physiol Renal Physiol, February 1, 1999; 276(2): F179 - F190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen Reduced AQP1, -2, and -3 levels in kidneys of rats with CRF induced by surgical reduction in renal mass Am J Physiol Renal Physiol, November 1, 1998; 275(5): F724 - F741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. N. Jonassen, S. Nielsen, S. Christensen, and J. S. Petersen Decreased vasopressin-mediated renal water reabsorption in rats with compensated liver cirrhosis Am J Physiol Renal Physiol, August 1, 1998; 275(2): F216 - F225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Christensen, D. Marples, U. B. Jensen, J. Frokiaer, D. Sheikh-Hamad, M. Knepper, and S. Nielsen Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution Am J Physiol Renal Physiol, August 1, 1998; 275(2): F285 - F297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sands, F. X. Flores, A. Kato, M. A. Baum, E. M. Brown, D. T. Ward, S. C. Hebert, and H. W. Harris Vasopressin-elicited water and urea permeabilities are altered in IMCD in hypercalcemic rats Am J Physiol Renal Physiol, May 1, 1998; 274(5): F978 - F985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Terris, D. Andersen, C. Ecelbarger, J. Frokiar, T. Jonassen, D. Marples, M. A. Knepper, and J. S. Petersen Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct PNAS, May 13, 1997; 94(10): 5450 - 5455. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
