AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 270: F682-F690, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schelling, J. R.
Right arrow Articles by Dubyak, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schelling, J. R.
Right arrow Articles by Dubyak, G. R.

AJP - Renal Physiology, Vol 270, Issue 4 682-F690, Copyright © 1996 by American Physiological Society

ARTICLES

Annexin II inhibition of G protein-regulated inositol trisphosphate formation in rat aortic smooth muscle

J. R. Schelling, D. J. Gentry and G. R. Dubyak
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.

Vasoconstrictor hormones contribute to the pathogenesis of hypertension through intracellular signals that stimulate vascular smooth muscle (VSMC) contraction and/or growth. We previously showed that the glucocorticoid dexamethasone (DEX) inhibited angiotensin II-stimulated inositol trisphosphate (IP3) formation in VSMC, but the mechanism of inhibition is not known. Because glucocorticoids stimulate the expression of annexins and annexin II potently binds phosphoinositides, the role of DEX and annexin II in VSMC G protein-coupled phosphoinositide hydrolysis was investigated. DEX incubation blunted increases in guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S)-stimulated IP3 generation and angiotensin II-induced intracellular Ca2+ mobilization but stimulated elevations in VSMC annexin II content. VSMC incubation with exogenous purified annexin II resulted in concentration-dependent decreases in GTP gamma S-stimulated IP3 formation. In DEX-treated cells, exogenous annexin II did not further diminish GTP gamma S-stimulated IP3 formation, suggesting that endogenous annexin II may be a mediator of DEX-induced inhibition of G protein-coupled IP3 generation. These data represent the first direct evidence of G protein-dependent phosphoinositide hydrolysis regulation by glucocorticoids or annexins. We speculate that annexin II may play a role in the pathogenesis of hypertension through stimulation of VSMC growth.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. L. Wu, S. Khan, S. Lakhe-Reddy, L. Wang, G. Jarad, R. T. Miller, M. Konieczkowski, A. M. Brown, J. R. Sedor, and J. R. Schelling
Renal tubular epithelial cell apoptosis is associated with caspase cleavage of the NHE1 Na+/H+ exchanger
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F829 - F839.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Trouve, S. Legot, I. Belikova, F. Marotte, D. Benevolensky, F. Russo-Marie, J.-L. Samuel, and D. Charlemagne
Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs
Am J Physiol Heart Circ Physiol, April 1, 1999; 276(4): H1159 - H1166.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Ushio-Fukai, K. K. Griendling, M. Akers, P. R. Lyons, and R. W. Alexander
Temporal Dispersion of Activation of Phospholipase C-beta 1 and -gamma Isoforms by Angiotensin II in Vascular Smooth Muscle Cells. ROLE OF alpha q/11, alpha 12, AND beta gamma G PROTEIN SUBUNITS
J. Biol. Chem., July 31, 1998; 273(31): 19772 - 19777.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online