AJP - Renal Physiology, Vol 270, Issue 5 718-F727, Copyright © 1996 by American Physiological Society

ARTICLES

Blockade of chloride channels by DIDS stimulates renin release and inhibits contraction of afferent arterioles

B. L. Jensen and O. Skott
Department of Medical Physiology, University of Copenhagen, Denmark.

Calcium-activated chloride channels have been proposed to control renin release from juxtaglomerular cells and to be involved in the excitation-contraction coupling of the renal afferent arteriole. The hypothesis was tested on renin release from rat glomeruli and in microperfused rabbit afferent arterioles with the chloride channel blocker 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). Renin secretion was equally enhanced by omission of extracellular calcium and by addition of 0.5 mM DIDS. The inhibitory effect of calcium was blocked by DIDS. The stimulatory effects of low calcium [with or without ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] and DIDS were not additive. In the absence of chloride, basal renin release was suppressed and the stimulatory effect of DIDS was abolished. The DIDS-induced enhancement of renin release was not dependent on bicarbonate. Norepinephrine (5 x 10(-7)-1 x 10(-6) M) and angiotensin II (1 x 10(-8)-10(-6) M) evoked reversible and dose-dependent contractions of microperfused rabbit afferent arterioles. DIDS (0.5 mM) did not affect the basal diameter of the arterioles but strongly inhibited the response to angiotensin II and attenuated the duration of the contractile response to norepinephrine. The results support the hypothesis that DIDS-sensitive calcium-activated chloride channels are involved in regulation of renin release and in the afferent arteriolar contraction after angiotensin II but do not play a pivotal role in the response to norepinephrine.

This article has been cited by other articles:

				E. C. Claud, J. Lu, X. Q. Wang, M. Abe, E. O. Petrof, J. Sun, D. J. Nelson, J. Marks, and T. Jilling Platelet-activating factor-induced chloride channel activation is associated with intracellular acidosis and apoptosis of intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1191 - G1200. [Abstract] [Full Text] [PDF]

				L. Balasubramanian, A. Ahmed, C.-M. Lo, J. S. K. Sham, and K.-P. Yip Integrin-mediated mechanotransduction in renal vascular smooth muscle cells: activation of calcium sparks Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1586 - R1594. [Abstract] [Full Text] [PDF]

				A. J. Fuller, B. C. Hauschild, R. Gonzalez-Villalobos, M. S. Awayda, J. D. Imig, E. W. Inscho, and L. G. Navar Calcium and chloride channel activation by angiotensin II-AT1 receptors in preglomerular vascular smooth muscle cells Am J Physiol Renal Physiol, October 1, 2005; 289(4): F760 - F767. [Abstract] [Full Text] [PDF]

				J. Steendahl, N.-H. Holstein-Rathlou, C. M. Sorensen, and M. Salomonsson Effects of chloride channel blockers on rat renal vascular responses to angiotensin II and norepinephrine Am J Physiol Renal Physiol, February 1, 2004; 286(2): F323 - F330. [Abstract] [Full Text] [PDF]

				M. Salomonsson, F. Gustafsson, D. Andreasen, B. L. Jensen, and N.-H. Holstein-Rathlou Local electric stimulation causes conducted calcium response in rat interlobular arteries Am J Physiol Renal Physiol, September 1, 2002; 283(3): F473 - F480. [Abstract] [Full Text] [PDF]

				Z. Zhang, J. M. C. Huang, M. R. Turner, K. L. Rhinehart, and T. L. Pallone Role of chloride in constriction of descending vasa recta by angiotensin II Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1878 - R1886. [Abstract] [Full Text] [PDF]

				R. W. Fallet, J. P. Bast, K. Fujiwara, N. Ishii, S. C. Sansom, and P. K. Carmines Influence of Ca2+-activated K+ channels on rat renal arteriolar responses to depolarizing agonists Am J Physiol Renal Physiol, April 1, 2001; 280(4): F583 - F591. [Abstract] [Full Text] [PDF]

				K. E. PURDY and W. J. ARENDSHORST Iloprost Inhibits Inositol-1,4,5-Trisphosphate-Mediated Calcium Mobilization Stimulated by Angiotensin II in Cultured Preglomerular Vascular Smooth Muscle Cells J. Am. Soc. Nephrol., January 1, 2001; 12(1): 19 - 28. [Abstract] [Full Text]

				S. Gambaryan, C. Wagner, A. Smolenski, U. Walter, W. Poller, W. Haase, A. Kurtz, and S. M. Lohmann Endogenous or overexpressed cGMP-dependent protein kinases inhibit cAMP-dependent renin release from rat isolated perfused kidney, microdissected glomeruli, and isolated juxtaglomerular cells PNAS, July 21, 1998; 95(15): 9003 - 9008. [Abstract] [Full Text] [PDF]