| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 271, Issue 2 446-F450, Copyright © 1996 by American Physiological Society
ARTICLES |
T. Wang, A. L. Egbert Jr, T. Abbiati, P. S. Aronson and G. Giebisch
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520-8026, USA.
We have previously demonstrated that formate and oxalate stimulate volume absorption in the rat proximal tubule, consistent with Cl-/formate and Cl-/oxalate exchange process across the apical membrane. To sustain Cl- absorption by these processes requires mechanisms for recycling formate and oxalate from lumen to cell. The aims of the present study were to characterize these mechanisms of formate and oxalate recycling. Proximal tubules and peritubular capillaries were simultaneously microperfused in the rat kidney in situ. Serum formate concentration was determined to be 56.5 +/- 7.7 microM. Addition of 5, 50, and 500 microM formate to both luminal and capillary perfusates significantly increased net Cl- absorption (Jcl) by 26, 26, and 46%, respectively. Jcl was stimulated 38% by 1 microM oxalate added to the perfusates. Removal of sulfate completely prevented the stimulation of Jcl by 1 microM oxalate but had no effect on the stimulation of Jcl by formate. Luminal addition of the Na+/H+ exchange inhibitor ethylisopropylamiloride completely blocked the stimulation of Jcl by 50 microM formate but had no effect on stimulation by oxalate. We conclude that physiological concentrations of formate and oxalate markedly stimulate Cl- and fluid absorption in the rat proximal convoluted tubule. Whereas formate recycling most likely involves Na+/H+ exchange in parallel with H(+)-coupled formate entry, oxalate recycling involves sodium-sulfate cotransport in parallel with sulfate/oxalate exchange.
This article has been cited by other articles:
|
|
 |
|
  H. A. Hassan, S. Mentone, L. P. Karniski, V. M. Rajendran, and P. S. Aronson Regulation of anion exchanger Slc26a6 by protein kinase C Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1485 - C1492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Freel, M. Hatch, M. Green, and M. Soleimani Ileal oxalate absorption and urinary oxalate excretion are enhanced in Slc26a6 null mice Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G719 - G728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Dudas, S. Mentone, C. F. Greineder, D. Biemesderfer, and P. S. Aronson Immunolocalization of anion transporter Slc26a7 in mouse kidney Am J Physiol Renal Physiol, April 1, 2006; 290(4): F937 - F945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, T. Wang, S. Petrovic, B. Tuo, B. Riederer, S. Barone, J. N. Lorenz, U. Seidler, P. S. Aronson, and M. Soleimani Renal and intestinal transport defects in Slc26a6-null mice Am J Physiol Cell Physiol, April 1, 2005; 288(4): C957 - C965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Petrovic, S. Barone, A. M. Weinstein, and M. Soleimani Activation of the apical Na+/H+ exchanger NHE3 by formate: a basis of enhanced fluid and electrolyte reabsorption by formate in the kidney Am J Physiol Renal Physiol, August 1, 2004; 287(2): F336 - F346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Weinstein Mathematical models of renal fluid and electrolyte transport: acknowledging our uncertainty Am J Physiol Renal Physiol, May 1, 2003; 284(5): F871 - F884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Karniski, T. Wang, L. A. Everett, E. D. Green, G. Giebisch, and P. S. Aronson Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein Am J Physiol Renal Physiol, November 1, 2002; 283(5): F952 - F956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Xie, R. Welch, A. Mercado, M. F. Romero, and D. B. Mount Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1 Am J Physiol Renal Physiol, October 1, 2002; 283(4): F826 - F838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Jiang, I. I. Grichtchenko, W. F. Boron, and P. S. Aronson Specificity of Anion Exchange Mediated by Mouse Slc26a6 J. Biol. Chem., September 6, 2002; 277(37): 33963 - 33967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, C.-L. Yang, T. Abbiati, G. E. Shull, G. Giebisch, and P. S. Aronson Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule Am J Physiol Renal Physiol, August 1, 2001; 281(2): F288 - F292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Karniski, M. Lotscher, M. Fucentese, H. Hilfiker, J. Biber, and H. Murer Immunolocalization of sat-1 sulfate/oxalate/bicarbonate anion exchanger in the rat kidney Am J Physiol Renal Physiol, July 1, 1998; 275(1): F79 - F87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, A. L. Egbert Jr., P. S. Aronson, and G. Giebisch Effect of metabolic acidosis on NaCl transport in the proximal tubule Am J Physiol Renal Physiol, June 1, 1998; 274(6): F1015 - F1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Karniski Effects of sulfate and chloride on three separate oxalate transporters reconstituted from rabbit renal cortex Am J Physiol Renal Physiol, January 1, 1998; 274(1): F189 - F196. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
