AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 273: F224-F233, 1997;
0363-6127/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lieske, J. C.
Right arrow Articles by Toback, F. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lieske, J. C.
Right arrow Articles by Toback, F. G.

AJP - Renal Physiology, Vol 273, Issue 2 224-F233, Copyright © 1997 by American Physiological Society

ARTICLES

Adhesion of hydroxyapatite crystals to anionic sites on the surface of renal epithelial cells

J. C. Lieske, R. Norris and F. G. Toback
Department of Medicine, University of Chicago, Illinois 60637, USA.

Adhesion of microcrystals that nucleate in tubular fluid to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones, 20% of which contain hydroxyapatite (HA). HA crystals bound rapidly to monolayer cultures of monkey kidney epithelial cells (BSC-1 line), used to model the surface of the nephron, in a concentration-dependent manner. Adhesion was blocked by diverse polyanions including heparin, pentosan polysulfate, polyaspartate, and polyglutamate, as well as many found in tubular fluid such as chondroitin sulfates A and B, heparan sulfate, citrate, nephrocalcin, and osteopontin. The polycations cetylpyridinium chloride and cationized ferritin, as well as the cationic dyes alcian blue, polyethylenimine, and brilliant blue R, also inhibited adhesion of HA crystals, as did specific lectins including Triticum vulgaris (wheat germ agglutinin). Anions that inhibited adhesion of crystals appeared to act on the crystal surface, whereas cations and lectins exerted their effect on the cell. Treatment of cells with neuraminidase inhibited binding of crystals, suggesting that anionic cell surface sialic acid residues function as HA crystal receptor sites that can be blocked by specific cations or lectins. Adherence of HA crystals to cells of another renal line (MDCK) and, to 3T3 fibroblasts was also inhibited by heparin, polyaspartate, alcian blue, and T vulgaris lectin, suggesting that these crystals bind to analogous molecules on the surface of different types of cells. These results suggests that the structure, quantity, and/or function of soluble anions in tubular fluid, as well as those anchored to the cell surface, could be critical determinants of HA crystal retention in the nephron and the subsequent formation of a renal stone.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
R. M. Koka, E. Huang, and J. C. Lieske
Adhesion of uric acid crystals to the surface of renal epithelial cells
Am J Physiol Renal Physiol, June 1, 2000; 278(6): F989 - F998.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. L. HUDKINS, C. M. GIACHELLI, YAN CUI, W. G. COUSER, R. J. JOHNSON, and C. E. ALPERS
Osteopontin Expression in Fetal and Mature Human Kidney
J. Am. Soc. Nephrol., March 1, 1999; 10(3): 444 - 457.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. F. Verkoelen, B. G. Van Der Boom, A. B. Houtsmuller, F. H. Schroder, and J. C. Romijn
Increased calcium oxalate monohydrate crystal binding to injured renal tubular epithelial cells in culture
Am J Physiol Renal Physiol, May 1, 1998; 274(5): F958 - F965.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online