AJP - Renal Physiology, Vol 273, Issue 5 674-F679, Copyright © 1997 by American Physiological Society

ARTICLES

Inhibition of PAH transport by parathyroid hormone in OK cells: involvement of protein kinase C pathway

J. Nagai, I. Yano, Y. Hashimoto, M. Takano and K. Inui
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.

We have previously shown that the p-aminohippurate (PAH) transport system in OK kidney epithelial cell line is under the regulatory control of protein kinase C. Parathyroid hormone (PTH) could activate protein kinase C, as well as protein kinase A, in OK cells. In the present study, the effect of PTH on PAH transport was studied in OK cells. PTH inhibited the transcellular transport of PAH from the basal to the apical side, as well as the accumulation of PAH in OK cells. Basolateral PAH uptake was inhibited by PTH in a dose- and time-dependent manner. Protein kinase A activators did not affect the transcellular transport or the accumulation of PAH. The PTH-induced inhibition of the accumulation of PAH was blocked by a protein kinase C inhibitor staurosporine. These results suggest that PTH inhibits the PAH transport in OK cells and that the messenger system mediated by protein kinase C, not protein kinase A, plays an important role in the regulation of PAH transport by PTH.

This article has been cited by other articles:

				K. E. Wever, R. Masereeuw, D. S. Miller, X. M. Hang, and G. Flik Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport Am J Physiol Renal Physiol, January 1, 2007; 292(1): F38 - F46. [Abstract] [Full Text] [PDF]

				S. Soodvilai, S. H. Wright, W. H. Dantzler, and V. Chatsudthipong Involvement of tyrosine kinase and PI3K in the regulation of OAT3-mediated estrone sulfate transport in isolated rabbit renal proximal tubules Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1057 - F1064. [Abstract] [Full Text] [PDF]

				S. Soodvilai, V. Chatsudthipong, K. K. Evans, S. H. Wright, and W. H. Dantzler Acute regulation of OAT3-mediated estrone sulfate transport in isolated rabbit renal proximal tubules Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1021 - F1029. [Abstract] [Full Text] [PDF]

				S. H. Wright and W. H. Dantzler Molecular and Cellular Physiology of Renal Organic Cation and Anion Transport Physiol Rev, July 1, 2004; 84(3): 987 - 1049. [Abstract] [Full Text] [PDF]

				C. Sauvant, D. Hesse, H. Holzinger, K. K. Evans, W. H. Dantzler, and M. Gekle Action of EGF and PGE2 on basolateral organic anion uptake in rabbit proximal renal tubules and hOAT1 expressed in human kidney epithelial cells Am J Physiol Renal Physiol, April 1, 2004; 286(4): F774 - F783. [Abstract] [Full Text] [PDF]

				G. You, K. Kuze, R. A. Kohanski, K. Amsler, and S. Henderson Regulation of mOAT-mediated Organic Anion Transport by Okadaic Acid and Protein Kinase C in LLC-PK1 Cells J. Biol. Chem., March 31, 2000; 275(14): 10278 - 10284. [Abstract] [Full Text] [PDF]

				R. Lu, B. S. Chan, and V. L. Schuster Cloning of the human kidney PAH transporter: narrow substrate specificity and regulation by protein kinase C Am J Physiol Renal Physiol, February 1, 1999; 276(2): F295 - F303. [Abstract] [Full Text] [PDF]

				C. Sauvant, S. Silbernagl, and M. Gekle Exposure to Ochratoxin A Impairs Organic Anion Transport in Proximal-Tubule-Derived Opossum Kidney Cells J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 13 - 20. [Abstract] [Full Text]

				C. Sauvant, H. Holzinger, and M. Gekle Modulation of the Basolateral and Apical Step of Transepithelial Organic Anion Secretion in Proximal Tubular Opossum Kidney Cells. ACUTE EFFECTS OF EPIDERMAL GROWTH FACTOR AND MITOGEN-ACTIVATED PROTEIN KINASE J. Biol. Chem., April 27, 2001; 276(18): 14695 - 14703. [Abstract] [Full Text] [PDF]